Document Detail

Epidermal growth factor receptor-coamplified and overexpressed protein (VOPP1) is a putative oncogene in gastric cancer.
MedLine Citation:
PMID:  25398664     Owner:  NLM     Status:  Publisher    
MicroRNAs are found to play an important role in gastric cancer. Reduced expression of microRNA-218 (miR-218) is of key interest. The target gene of microRNA-218, epidermal growth factor receptor-coamplified and overexpressed protein (ECOP) encoded by the VOPP1 gene, has been implicated in tumorigenesis. However, few studies on expression and function of ECOP in gastric cancer have been reported. ECOP expression was determined in matched normal and gastric adenocarcinoma tissue specimens by immunohistochemistry and western blot. Subsequently, ectopic overexpression and RNAi-mediated silencing of VOPP1 was effected in the human gastric cancer cell line, AGS. Proliferation and migration of parental, VOPP1 overexpressing and VOPP1-silenced AGS cells were evaluated by cell proliferation assay and scratch wound-healing motility assay. Finally, intracellular localization of ECOP in AGS cells was assessed by green fluorescent protein tagging and fluorescent microscopy. Western blot and immunohistochemistry showed overexpression of ECOP in gastric adenocarcinoma tissues compared to matched normal tissue specimens. Ectopic overexpression and RNAi-mediated silencing of VOPP1 promoted and inhibited, respectively, cell proliferation and migration in AGS cells. Intracellular localization of ECOP in perinuclear lysosomes mimicked colocalization earlier reported for other cancerous cells. VOPP1 is overexpressed in gastric adenocarcinoma, which is involved in promoting cell proliferation and migration and thus might serve as a putative oncogene.
Caiping Gao; Minghui Pang; Zhou Zhou; Size Long; Dandan Dong; Jiyun Yang; Mei Cao; Chumin Zhang; Shengxi Han; Liangping Li
Related Documents :
25325304 - Silencing the wild-type and mutant k-ras increases the resistance to 5-flurouracil in h...
25219824 - Synergistic in vitro anti-tumor effect of letrozole and everolimus on human endometrial...
25322694 - Induction of autophagy contributes to cisplatin resistance in human ovarian cancer cells.
231114 - Granular cell myoblastoma of the vulva.
1684044 - Changes in the levels of inositol lipids and phosphates during the differentiation of h...
9125454 - Development of contrast sensitivity and temporal-frequency selectivity in primate later...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-11-15
Journal Detail:
Title:  Clinical and experimental medicine     Volume:  -     ISSN:  1591-9528     ISO Abbreviation:  Clin. Exp. Med.     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-11-15     Completed Date:  -     Revised Date:  2014-11-16    
Medline Journal Info:
Nlm Unique ID:  100973405     Medline TA:  Clin Exp Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Conjunctival squamous cell neoplasia: the Liverpool Ocular Oncology Centre experience.
Next Document:  Rethinking 'Efficacy': Ritual Healing and Trance in the Mahanubhav Shrines in India.