Document Detail

Epidermal growth factor and insulin-like growth factor-1 preserve cell viability in the absence of protein synthesis.
MedLine Citation:
PMID:  8463188     Owner:  NLM     Status:  MEDLINE    
Prolonged exposure of cells to the potent protein synthesis inhibitor cycloheximide (CHX) terminates in cell death. In the present study we investigated the effect of epidermal growth factor (EGF), insulin-like growth factor-1 (IGF-1), and insulin on cell death induced by CHX in the human cancerous cell lines MDA-231 and MCF-7 (breast), KB (oral epidermoid), HEP-2 (larynx epidermoid), and SW-480 (colon), and correlated this effect to the inhibition rate of protein synthesis. Cell death was evaluated by measuring either dead cells by trypan blue dye exclusion test or by the release of lactic dehydrogenase into the culture medium. CHX was shown to induce cell death in a concentration (1 to 60 micrograms/ml) and time (24 to 72 h)-dependent manner in each of the five cell lines. EGF at physiologic concentrations (2 to 40 ng/ml) reduced cell death close to control level (without CHX) in the cell lines HEP-2, KB, MDA-231, and SW-480, but had almost no effect on cell death in the MCF-7 cells. IGF-1 at physiologic concentrations (2 to 40 ng/ml) reduced cell death nearly to control level in the MCF-7 cells, but had only a partial effect in the other four cell lines. Insulin at supraphysiologic concentration (10,000 ng/ml) mimicked the effect of IGF-1 in each of the cell lines. CHX at concentrations that induced about 60% cell death, inhibited about 90% of protein synthesis as measured by [3H]leucine incorporation. Protein synthesis remained inhibited although cell viability was preserved by EGF or IGF-1. These results indicated that the mechanism by which EGF or IGF-1 preserve cell viability does not require new protein synthesis and may be mediated via a posttranslational modification effect.
A Geier; R Hemi; M Haimson; R Beery
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  29A     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-05-04     Completed Date:  1993-05-04     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  231-4     Citation Subset:  IM    
Institute of Endocrinology, Chaim Sheba Medical Center, Tel Hashomer, Israel.
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MeSH Terms
Breast Neoplasms
Cell Survival / drug effects*
Colonic Neoplasms
Cycloheximide / antagonists & inhibitors
Epidermal Growth Factor / pharmacology*
Insulin-Like Growth Factor I / pharmacology*
Leucine / metabolism
Mouth Neoplasms
Protein Biosynthesis
Tumor Cells, Cultured / drug effects*
Reg. No./Substance:
61-90-5/Leucine; 62229-50-9/Epidermal Growth Factor; 66-81-9/Cycloheximide; 67763-96-6/Insulin-Like Growth Factor I

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