Document Detail

Epidermal growth factor induces the accumulation of calpactin II on the cell surface during membrane ruffling.
MedLine Citation:
PMID:  2136806     Owner:  NLM     Status:  MEDLINE    
Confluent and proliferatively quiescent T51B rat liver epithelial cells provide a cellular model for the study of epidermal growth factor (EGF) effects in non-neoplastic cells. Immunoreactive calpactin II, a well-known substrate for EGF-receptor kinase, was found predominantly in the cytosol, although a second immunoreactive pool was found in a Triton X-100-extractable membrane fraction. Stimulation with EGF resulted in a rapid and transient (2-5 min) formation of ruffles at the cell surface and at the cell-cell contacts. Both calpactin II and filamentous actin were found co-localized at the membrane ruffles. Immunoprecipitations of membrane-bound calpactin II from 32P-labeled cells indicate a transient EGF-dependent phosphorylation of calpactin II correlating with membrane ruffling. These results suggest a temporal (2-5 min) function for calpactin II at the plasma membrane during the EGF-induced mitogenesis of T51B cells.
R Campos-Gonzalez; M Kanemitsu; A L Boynton
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell motility and the cytoskeleton     Volume:  15     ISSN:  0886-1544     ISO Abbreviation:  Cell Motil. Cytoskeleton     Publication Date:  1990  
Date Detail:
Created Date:  1990-02-09     Completed Date:  1990-02-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8605339     Medline TA:  Cell Motil Cytoskeleton     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  34-40     Citation Subset:  IM    
Cancer Research Center of Hawaii, University of Hawaii, Honolulu.
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MeSH Terms
Actins / analysis
Calcium-Binding Proteins / analysis*,  metabolism
Cell Line
Cell Membrane / analysis*,  drug effects,  ultrastructure
Cytosol / analysis
Epidermal Growth Factor / pharmacology*
Fluorescent Antibody Technique
Membrane Proteins / analysis*,  metabolism
Grant Support
Reg. No./Substance:
0/Actins; 0/Annexins; 0/Calcium-Binding Proteins; 0/Membrane Proteins; 62229-50-9/Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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