Document Detail

Epidermal growth factor-induced mobilization of a ganglioside-specific sialidase (NEU3) to membrane ruffles.
MedLine Citation:
PMID:  16765317     Owner:  NLM     Status:  MEDLINE    
Human ganglioside-specific sialidase, NEU3, localized at cell membranes is thought to regulate various biological processes at cell surfaces. We here explored functional subcellular localization of the sialidase by immunofluorescence and found accumulation at leading edges of cell membranes in the presence of serum in culture. In response to EGF, the sialidase redistributed rapidly to ruffling cell membranes of squamous carcinoma A431 cells and co-localized with Rac-1. NEU3 overexpression enhanced Rac-1 activation and cell migration as compared with controls in HeLa cells as well as in A431 cells. Consistent with co-localization with Rac-1 by immunofluorescence, NEU3 was found to co-precipitate with activated Rac bound to GST-PAK-1 fusion protein. NEU3 silencing by siRNA, in contrast, resulted in inhibition of Rac-1 activation. These results indicate that NEU3 is able to mobilize to membrane ruffles in response to growth stimuli and activate the Rac-1 signaling by co-localization with Rac-1, leading to increased cell motility.
Kazunori Yamaguchi; Keiko Hata; Tadashi Wada; Setsuko Moriya; Taeko Miyagi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-06-05
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  346     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-20     Completed Date:  2006-08-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  484-90     Citation Subset:  IM    
Division of Biochemistry, Miyagi Cancer Center Research Institute, Natori, Japan.
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MeSH Terms
Cell Line
Cell Membrane Structures / metabolism*
Cell Movement
Cercopithecus aethiops
Enzyme Activation
Epidermal Growth Factor / physiology*
Neuraminidase / biosynthesis,  genetics,  metabolism*
Protein Binding
Protein Transport
Protein-Serine-Threonine Kinases / metabolism
p21-Activated Kinases
rac1 GTP-Binding Protein / metabolism
Reg. No./Substance:
0/Pak1 protein, mouse; 62229-50-9/Epidermal Growth Factor; EC protein, human; EC Kinases; EC Kinases; EC protein, human; EC; EC GTP-Binding Protein

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