| Epidermal growth factor: the driving force in initiation of RPE cell proliferation. | |
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MedLine Citation:
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PMID: 21494877 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: To analyze whether epidermal growth factor (EGF) exerts regulatory effects on proliferation and differentiation in ARPE19 cells after different incubation periods (24 vs. 48 h) for obtaining ideal conditions for feasible rejuvenation and autologous transplantation of retinal pigment epithelial cells (RPE cells). METHODS: To evaluate gene expression patterns of RPE-specific differentiation and proliferation markers as well as transcriptional and translational changes of beta-catenin (ß-catenin)-signaling markers by fluorescence activated cell sorting (FACS) and reverse transcription - polymerase chain reaction (RT-PCR) after 24 h of EGF treatment. RESULTS: After 24 h of EGF treatment, a significant decrease of retinal pigment epithelium-specific protein 65 (RPE 65), cellular retinaldehyde-binding protein (CRALBP) and cytokeratin 18 in ARPE-19 cells was scaled. In addition, an increase of cyclin D1 expression and a significant decrease of glycogen synthase kinase-3beta (GSK-3ß) and beta-catenin (ß-catenin) were equally observed after 24 and 48 h of EGF treatment. Cell-cycle studies revealed an increase of ARPE cells in S-G2/M phase after 24 h of EGF treatment. CONCLUSIONS: Our data demonstrate the induction of proliferation and upregulation of the ß-catenin signaling pathway by EGF even after 24 h of incubation. As ideal cell culture conditions are essential for maintaining RPE-specific phenotypes, short incubation times enhance RPE cell quality for feasible rejuvenation and subsequent autologous transplantation of RPE cells. |
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Authors:
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Kerstin Steindl-Kuscher; Michael E Boulton; Paulina Haas; Astrid Dossenbach-Glaninger; Hans Feichtinger; Susanne Binder |
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Publication Detail:
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Type: Journal Article Date: 2011-04-15 |
Journal Detail:
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Title: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv für klinische und experimentelle Ophthalmologie Volume: 249 ISSN: 1435-702X ISO Abbreviation: Graefes Arch. Clin. Exp. Ophthalmol. Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-08-08 Completed Date: 2011-10-24 Revised Date: 2012-05-28 |
Medline Journal Info:
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Nlm Unique ID: 8205248 Medline TA: Graefes Arch Clin Exp Ophthalmol Country: Germany |
Other Details:
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Languages: eng Pagination: 1195-200 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Ludwig Boltzmann Institute for Retinology and Biomicroscopic Lasersurgery, Rudolf Foundation Clinic, Juchgasse 25, 1030 Vienna, Austria. kerstin.steindl@aon.at |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biological Markers Carrier Proteins / genetics, metabolism Cell Cycle Cell Differentiation / drug effects* Cell Line Cell Proliferation / drug effects* DNA Primers / chemistry Epidermal Growth Factor / pharmacology* Eye Proteins / genetics, metabolism Flow Cytometry Gene Expression Profiling Glycogen Synthase Kinase 3 / genetics, metabolism Humans Keratin-18 / genetics, metabolism RNA, Messenger / metabolism Retinal Pigment Epithelium / cytology*, metabolism Reverse Transcriptase Polymerase Chain Reaction beta Catenin / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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P30 EY021721/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/11-cis-retinal-binding protein; 0/Biological Markers; 0/CTNNB1 protein, human; 0/Carrier Proteins; 0/DNA Primers; 0/Eye Proteins; 0/Keratin-18; 0/RNA, Messenger; 0/beta Catenin; 62229-50-9/Epidermal Growth Factor; EC 2.7.11.1/glycogen synthase kinase 3 beta; EC 2.7.11.26/Glycogen Synthase Kinase 3; EC 3.1.1.64/retinoid isomerohydrolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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