Document Detail


Epidermal and dermal phospholipids of the human eyelid: a 31P nuclear magnetic resonance spectroscopy study.
MedLine Citation:
PMID:  9705160     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The phospholipids of the skin are difficult to quantify because they represent only a small fraction of the skin tissue. In this study, 31P nuclear magnetic resonance, which permits precise profiling of these phospholipids, was used to compare the phospholipids of upper eyelid epidermal and dermal lipid extracts (n = 13 profiles). Phospholipid profiles included alkylacylphosphatidylcholine (AAPC), dihydrosphingomyelin (DHSM), diphosphatidylglycerol (cardiolipin), ethanolamine plasmalogen (EPLAS), lysophosphatidylcholine, phosphatidic acid, phosphatidylcholine (PC), phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, sphingomyelin, and uncharacterized phospholipids (U1 and U2, particularly enriched in the epidermis). The computed phospholipid metabolic index (n = 86 indexes) findings can be summarized as follows: a lower content of the en-ol and ether phospholipids in the epidermis relative to the dermis, internal compensation among the component phospholipids so as to maintain the choline functional group ratio, and a greater concentration of hydroxyl-containing functional groups in the epidermis. A membrane index (fmem) value of -0.37 for the epidermis deviated considerably from the value of -0.06 characteristic of living membranes and the dermis. The production of the reduced phosphatides, EPLAS and AAPC, indicates the use of alternative pathways between the two tissues. Relative to the dermis, increased PC in the epidermis coupled with decreased DHSM, EPLAS, and AAPC are factors enabling the epidermis of eyelid tissue to be an effective water barrier.
Authors:
J V Greiner; T Glonek; D J Townsend; S L Hearn
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Archives of dermatological research     Volume:  290     ISSN:  0340-3696     ISO Abbreviation:  Arch. Dermatol. Res.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-11-06     Completed Date:  1998-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8000462     Medline TA:  Arch Dermatol Res     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  298-305     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.
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MeSH Terms
Descriptor/Qualifier:
Dermis / chemistry*
Epidermis / chemistry*
Eyelids / chemistry*
Humans
Magnetic Resonance Spectroscopy
Phospholipids / analysis*,  chemistry
Phosphorus Isotopes
Chemical
Reg. No./Substance:
0/Phospholipids; 0/Phosphorus Isotopes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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