Document Detail

Epidermal cells of a symbiosis-defective mutant of Lotus japonicus show altered cytoskeleton organisation in the presence of a mycorrhizal fungus.
MedLine Citation:
PMID:  11926066     Owner:  NLM     Status:  MEDLINE    
The influence of the mycorrhizal fungus Gigaspora margarita on cytoskeleton organisation in epidermal cells of Lotus japonicus roots was compared between plants of the wild type Gifu and the mutant Ljsym4-2, in which the fungus is confined to the epidermal cells. Immunofluorescence labelling of plant microtubules and microfilaments showed only limited alterations in the peripheral cytoskeleton of epidermal cells during early stages of fungal interaction with the wild type. Later, microtubules and microfilaments enveloped the growing hypha, while the host cell nucleus moved close to the fungus. In contrast, epidermal cells of the mutant responded with disorganisation and disassembly of microtubules and microfilaments before and during fungal penetration attempts. The fungus penetrated only as far as to epidermal cells, whose cytoplasm became devoid of tubulin and actin, suggesting cell death. The close relationship between host cytoskeleton organisation and compatibility with the fungus suggests that a functional Ljsym4 gene is necessary for correct reorganisation of the epidermal cell cytoskeleton in the presence of the fungus and for avoiding hypersensitivity-like reactions.
A Genre; P Bonfante
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Protoplasma     Volume:  219     ISSN:  0033-183X     ISO Abbreviation:  Protoplasma     Publication Date:  2002 Feb 
Date Detail:
Created Date:  2002-04-02     Completed Date:  2002-10-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  43-50     Citation Subset:  IM    
Dipartimento di Biologia Vegetale, Università di Torino, Centro di Studio sulla Micologia del Terreno, Consiglio Nazionale delle Ricerche, Torino, Italy.
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MeSH Terms
Cell Death
Cell Survival
Cytoskeleton / immunology,  microbiology*,  pathology*
Fungi / isolation & purification,  physiology*
Lotus / cytology*,  genetics,  microbiology*
Mutation / genetics*
Plant Epidermis / cytology,  microbiology
Plant Roots / cytology,  microbiology
Symbiosis / genetics*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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