Document Detail

Epidemiologic Study of Malassezia Yeasts in Seborrheic Dermatitis Patients by the Analysis of 26S rDNA PCR-RFLP.
MedLine Citation:
PMID:  20548904     Owner:  NLM     Status:  PubMed-not-MEDLINE    
BACKGROUND: This case-control study concerns a molecular biological method based on the data gathered from a group of Korean subjects to examine the distribution of Malassezia yeasts in seborrheic dermatitis (SD) patients. Cultures for Malassezia yeasts were taken from the foreheads, cheeks and chests of 60 patients with SD and in 60 healthy controls of equivalent age.
OBJECTIVE: The purpose of this study is to identify the relationship between certain species of Malassezia and SD. This was done by analyzing the differences in the distribution of Malassezia species in terms of age and body parts of the host with healthy controls.
METHODS: 26S rDNA PCR-RFLP, a fast and accurate molecular biological method, was used to overcome the limits of morphological and biochemical methods.
RESULTS: The positive Malassezia culture rate was 51.7% in patients with SD, which was lower than that of healthy adults (63.9%). M. restricta was dominant in patients with SD (19.5%). Likewise, M. restricta was identified as a common species (20.5%) in healthy controls. In the ages 31~40, M. restricta was found to be the most common species (31.6%) among SD patients.
CONCLUSION: According to the results of the study, the most frequently isolated species was M. restricta (19.5%) in patients with SD. There was no statistically significant difference in the distribution of Malassezia species between the SD patients and healthy control groups.
Byung Ho Oh; Yang Won Lee; Yong Beom Choe; Kyu Joong Ahn
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Publication Detail:
Type:  Journal Article     Date:  2010-05-17
Journal Detail:
Title:  Annals of dermatology     Volume:  22     ISSN:  2005-3894     ISO Abbreviation:  Ann Dermatol     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-06-15     Completed Date:  2011-07-14     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  8916577     Medline TA:  Ann Dermatol     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  149-55     Citation Subset:  -    
Department of Dermatology, Konkuk University School of Medicine, Seoul, Korea.
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