Document Detail


Epicatechin and catechin in cocoa inhibit amyloid beta protein induced apoptosis.
MedLine Citation:
PMID:  15740021     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To elucidate additional health benefits of cocoa phytochemicals on the neurotoxicity induced by amyloid beta protein (Abeta), PC12 cells were treated with toxic peptide (Abeta(25)(-)(35)) and the effects of epicatechin, catechin, and cocoa were studied using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction, lactate dehydrogenase (LDH) release, and trypan blue exclusion methods. Significant increase in neuronal cell death was observed on PC12 cells treated with Abeta(25)(-)(35) (25 microM), while epicatechin and catechin and their mixture prevented the Abeta-induced neuronal cell death. Abeta treatment also led to the increased membrane instability of PC12 cells. The membrane protective effects of the phenolics determined by LDH release and trypan blue exclusion assays demonstrated that epicatechin, catechin, and their mixture protect cellular membrane from Abeta-induced cytotoxicity. In these three different cell viability assays, the mixture of epicatechin and catechin showed the highest protective effect and synergistic activity. The present results showed that the major flavonoids of cocoa, epicatechin and catechin, protect PC12 cells from Abeta-induced neurotoxicity, and suggest that cocoa may have anti-neurodegenerative effect in addition to other known chemopreventive effects.
Authors:
Ho Jin Heo; Chang Yong Lee
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  53     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-02     Completed Date:  2005-04-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1445-8     Citation Subset:  IM    
Affiliation:
Department of Food Science and Technology, Cornell University, Geneva, New York 14456, USA.
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MeSH Terms
Descriptor/Qualifier:
Amyloid beta-Protein / pharmacology*
Animals
Apoptosis / drug effects*
Cacao / chemistry*
Catechin / pharmacology*
Cell Membrane / drug effects
Neurodegenerative Diseases / prevention & control
Neurons / drug effects,  ultrastructure
PC12 Cells
Rats
Chemical
Reg. No./Substance:
0/Amyloid beta-Protein; 154-23-4/Catechin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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