| Epicatechin and catechin in cocoa inhibit amyloid beta protein induced apoptosis. | |
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MedLine Citation:
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PMID: 15740021 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To elucidate additional health benefits of cocoa phytochemicals on the neurotoxicity induced by amyloid beta protein (Abeta), PC12 cells were treated with toxic peptide (Abeta(25)(-)(35)) and the effects of epicatechin, catechin, and cocoa were studied using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction, lactate dehydrogenase (LDH) release, and trypan blue exclusion methods. Significant increase in neuronal cell death was observed on PC12 cells treated with Abeta(25)(-)(35) (25 microM), while epicatechin and catechin and their mixture prevented the Abeta-induced neuronal cell death. Abeta treatment also led to the increased membrane instability of PC12 cells. The membrane protective effects of the phenolics determined by LDH release and trypan blue exclusion assays demonstrated that epicatechin, catechin, and their mixture protect cellular membrane from Abeta-induced cytotoxicity. In these three different cell viability assays, the mixture of epicatechin and catechin showed the highest protective effect and synergistic activity. The present results showed that the major flavonoids of cocoa, epicatechin and catechin, protect PC12 cells from Abeta-induced neurotoxicity, and suggest that cocoa may have anti-neurodegenerative effect in addition to other known chemopreventive effects. |
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Authors:
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Ho Jin Heo; Chang Yong Lee |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 53 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2005 Mar |
Date Detail:
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Created Date: 2005-03-02 Completed Date: 2005-04-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1445-8 Citation Subset: IM |
Affiliation:
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Department of Food Science and Technology, Cornell University, Geneva, New York 14456, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amyloid beta-Protein
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pharmacology* Animals Apoptosis / drug effects* Cacao / chemistry* Catechin / pharmacology* Cell Membrane / drug effects Neurodegenerative Diseases / prevention & control Neurons / drug effects, ultrastructure PC12 Cells Rats |
| Chemical | |
Reg. No./Substance:
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0/Amyloid beta-Protein; 154-23-4/Catechin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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