Document Detail

Epi-laser in situ keratomileusis: comparative evaluation of epithelial separation with 3 microkeratomes.
MedLine Citation:
PMID:  18812130     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To evaluate the cleavage plane, corneal cytoarchitecture, and cell vitality of separated corneal epithelial sheets created with 3 commonly used microkeratomes. SETTING: Laboratories of the Regensburg University Medical Center, Regensburg, Germany. METHODS: Mechanical separation of the epithelial layer in 10 porcine eyes and 2 human eyes was performed with 3 different microkeratomes (Amadeus II, Zyoptix XP, Epivision). Five of 10 porcine corneas and the 2 human corneas treated with each microkeratome were processed for histology, electron microscopy, and immunohistochemistry. In 5 of 10 porcine corneas, the corneal epithelial sheets were separated from the globe and cell vitality was assessed with the trypan blue dye vitality test. RESULTS: A reproducible epithelial separation with a smooth surface was achieved in all eyes. The cleavage plane was located between the lamina lucida and the lamina densa. Damage to epithelial cells was mainly limited to the cut margins. CONCLUSIONS: Mechanical separation of the epithelial sheet in epithelial laser in situ keratomileusis (epi-LASIK) was safe and reproducible with all evaluated microkeratomes. Immunohistochemistry and electron microscopy showed the cleavage plane in epi-LASIK was between the basal epithelium and the basement membrane at the level of the lamina lucida.
Wolfgang A Herrmann; Jost Hillenkamp; Karsten Hufendiek; Philipp Prahs; Chris P Lohmann; Horst Helbig; Karin Kobuch
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of cataract and refractive surgery     Volume:  34     ISSN:  0886-3350     ISO Abbreviation:  J Cataract Refract Surg     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-24     Completed Date:  2008-12-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8604171     Medline TA:  J Cataract Refract Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1761-6     Citation Subset:  IM    
Department of Ophthalmology, Regensburg University Medical Center, Regensburg, Germany.
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MeSH Terms
Biological Markers / metabolism
Cell Adhesion Molecules / metabolism
Cell Survival
Collagen Type VII / metabolism
Coloring Agents / diagnostic use
Cornea / surgery
Epithelium, Corneal / cytology*,  metabolism,  ultrastructure
Immunoenzyme Techniques
Integrin beta4 / metabolism
Keratomileusis, Laser In Situ / methods*
Lasers, Excimer*
Surgical Flaps*
Trypan Blue / diagnostic use
Reg. No./Substance:
0/Biological Markers; 0/Cell Adhesion Molecules; 0/Collagen Type VII; 0/Coloring Agents; 0/Integrin beta4; 0/kalinin; 72-57-1/Trypan Blue

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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