| EphB receptors regulate dendritic spine morphogenesis through the recruitment/phosphorylation of focal adhesion kinase and RhoA activation. | |
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MedLine Citation:
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PMID: 16298995 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dendritic filopodia are small protrusions on the surface of neuronal dendrites that transform into dendritic spines upon synaptic contact with axon terminals. The formation of dendritic spines is a critical aspect of synaptic development. Dendritic spine morphogenesis is characterized by filopodia shortening followed by the formation of mature mushroom-shaped spines. Here we show that activation of the EphB receptor tyrosine kinases in cultured hippocampal neurons by their ephrinB ligands induces morphogenesis of dendritic filopodia into dendritic spines. This appears to occur through assembly of an EphB-associated protein complex that includes focal adhesion kinase (FAK), Src, Grb2, and paxillin and the subsequent activations of FAK, Src, paxillin, and RhoA. Furthermore, Cre-mediated knock-out of loxP-flanked fak or RhoA inhibition blocks EphB-mediated morphogenesis of dendritic filopodia. Finally, EphB-mediated RhoA activation is disrupted by FAK knock-down. These data suggest that EphB receptors are upstream regulators of FAK in dendritic filopodia and that FAK-mediated RhoA activation contributes to assembly of actin filaments in dendritic spines. |
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Authors:
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Michael L Moeller; Yang Shi; Louis F Reichardt; Iryna M Ethell |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2005-11-18 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 281 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-01-17 Completed Date: 2006-03-17 Revised Date: 2011-01-26 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1587-98 Citation Subset: IM |
Affiliation:
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Division of Biomedical Sciences, University of California Riverside, Riverside, California 92521, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dendritic Spines / ultrastructure* Embryo, Mammalian Focal Adhesion Protein-Tyrosine Kinases / metabolism* Hippocampus / ultrastructure Ligands Mice Neurons / cytology, ultrastructure Phosphorylation Receptors, Eph Family / physiology* rhoA GTP-Binding Protein / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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MH67121/MH/NIMH NIH HHS; NS19090/NS/NINDS NIH HHS; R01 MH067121-04/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Ligands; EC 2.7.10.1/Receptors, Eph Family; EC 2.7.10.2/Focal Adhesion Protein-Tyrosine Kinases; EC 3.6.5.2/rhoA GTP-Binding Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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