Document Detail

Ependymal ciliary dysfunction and reactive astrocytosis in a reorganized subventricular zone after stroke.
MedLine Citation:
PMID:  22414771     Owner:  NLM     Status:  MEDLINE    
Subventricular zone (SVZ) astrocytes and ependymal cells are both derived from radial glia and may have similar gliotic reactions after stroke. Diminishing SVZ neurogenesis worsens outcomes in mice, yet the effects of stroke on SVZ astrocytes and ependymal cells are poorly understood. We used mouse experimental stroke to determine if SVZ astrocytes and ependymal cells assume similar phenotypes and if stroke impacts their functions. Using lateral ventricular wall whole mount preparations, we show that stroke caused SVZ reactive astrocytosis, disrupting the neuroblast migratory scaffold. Also, SVZ vascular density and neural proliferation increased but apoptosis did not. In contrast to other reports, ependymal denudation and cell division was never observed. Remarkably, however, ependymal cells assumed features of reactive astrocytes post stroke, robustly expressing de novo glial fibrillary acidic protein, enlargening and extending long processes. Unexpectedly, stroke disrupted motile cilia planar cell polarity in ependymal cells. This suggested ciliary function was affected and indeed ventricular surface flow was slower and more turbulent post stroke. Together, these results demonstrate that in response to stroke there is significant SVZ reorganization with implications for both pathophysiology and therapeutic strategies.
Christopher C Young; Judith M van der Harg; Nicola J Lewis; Keith J Brooks; Alastair M Buchan; Francis G Szele
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-03-13
Journal Detail:
Title:  Cerebral cortex (New York, N.Y. : 1991)     Volume:  23     ISSN:  1460-2199     ISO Abbreviation:  Cereb. Cortex     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-05     Completed Date:  2013-07-29     Revised Date:  2014-03-12    
Medline Journal Info:
Nlm Unique ID:  9110718     Medline TA:  Cereb Cortex     Country:  United States    
Other Details:
Languages:  eng     Pagination:  647-59     Citation Subset:  IM    
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MeSH Terms
Cilia / pathology*
Disease Models, Animal
Ependyma / pathology*,  physiopathology
Gliosis / pathology*
Lateral Ventricles / pathology*,  physiopathology
Mice, 129 Strain
Stroke / cerebrospinal fluid,  pathology*,  physiopathology
Grant Support
G0500495//Medical Research Council; R01 NS-42253/NS/NINDS NIH HHS; //Medical Research Council

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