Document Detail


Eosinophils in the zebrafish: prospective isolation, characterization, and eosinophilia induction by helminth determinants.
MedLine Citation:
PMID:  20713961     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Eosinophils are granulocytic leukocytes implicated in numerous aspects of immunity and disease. The precise functions of eosinophils, however, remain enigmatic. Alternative models to study eosinophil biology may thus yield novel insights into their function. Eosinophilic cells have been observed in zebrafish but have not been thoroughly characterized. We used a gata2:eGFP transgenic animal to enable prospective isolation and characterization of zebrafish eosinophils, and demonstrate that all gata2(hi) cells in adult hematopoietic tissues are eosinophils. Although eosinophils are rare in most organs, they are readily isolated from whole kidney marrow and abundant within the peritoneal cavity. Molecular analyses demonstrate that zebrafish eosinophils express genes important for the activities of mammalian eosinophils. In addition, gata2(hi) cells degranulate in response to helminth extract. Chronic exposure to helminth- related allergens resulted in profound eosinophilia, demonstrating that eosinophil responses to allergens have been conserved over evolution. Importantly, infection of adult zebrafish with Pseudocapillaria tomentosa, a natural nematode pathogen of teleosts, caused marked increases in eosinophil number within the intestine. Together, these observations support a conserved role for eosinophils in the response to helminth antigens or infection and provide a new model to better understand how parasitic worms activate, co-opt, or evade the vertebrate immune response.
Authors:
Keir M Balla; Geanncarlo Lugo-Villarino; Jan M Spitsbergen; David L Stachura; Yan Hu; Karina Bañuelos; Octavio Romo-Fewell; Raffi V Aroian; David Traver
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-08-16
Journal Detail:
Title:  Blood     Volume:  116     ISSN:  1528-0020     ISO Abbreviation:  Blood     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-12     Completed Date:  2010-12-16     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3944-54     Citation Subset:  AIM; IM    
Affiliation:
Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093-0380, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Antigens, Helminth
Base Sequence
Cell Degranulation / immunology
DNA Primers / genetics
Enoplida Infections / blood,  immunology,  parasitology
Eosinophilia / etiology,  immunology,  parasitology
Eosinophils / cytology,  immunology,  parasitology,  physiology*
GATA2 Transcription Factor / genetics,  metabolism
Host-Parasite Interactions
Neutrophils / physiology
Trichuroidea / immunology,  pathogenicity
Zebrafish / blood*,  genetics,  immunology,  parasitology
Zebrafish Proteins / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
3R01DK074482-01/DK/NIDDK NIH HHS; 5P30CA23100-22S2/CA/NCI NIH HHS; 5T34GM08303/GM/NIGMS NIH HHS; ES00210/ES/NIEHS NIH HHS; ES011587/ES/NIEHS NIH HHS; ES013124/ES/NIEHS NIH HHS; ES03850/ES/NIEHS NIH HHS; GMO68524//PHS HHS; R01-AI056189/AI/NIAID NIH HHS; R01-DK074482/DK/NIDDK NIH HHS; RR12546/RR/NCRR NIH HHS; T32-HL086344/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Helminth; 0/DNA Primers; 0/GATA2 Transcription Factor; 0/GATA2 protein, zebrafish; 0/Zebrafish Proteins
Comments/Corrections
Comment In:
Blood. 2010 Nov 11;116(19):3692-3   [PMID:  21071614 ]

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