| Eosinophils in the zebrafish: prospective isolation, characterization, and eosinophilia induction by helminth determinants. | |
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MedLine Citation:
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PMID: 20713961 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Eosinophils are granulocytic leukocytes implicated in numerous aspects of immunity and disease. The precise functions of eosinophils, however, remain enigmatic. Alternative models to study eosinophil biology may thus yield novel insights into their function. Eosinophilic cells have been observed in zebrafish but have not been thoroughly characterized. We used a gata2:eGFP transgenic animal to enable prospective isolation and characterization of zebrafish eosinophils, and demonstrate that all gata2(hi) cells in adult hematopoietic tissues are eosinophils. Although eosinophils are rare in most organs, they are readily isolated from whole kidney marrow and abundant within the peritoneal cavity. Molecular analyses demonstrate that zebrafish eosinophils express genes important for the activities of mammalian eosinophils. In addition, gata2(hi) cells degranulate in response to helminth extract. Chronic exposure to helminth- related allergens resulted in profound eosinophilia, demonstrating that eosinophil responses to allergens have been conserved over evolution. Importantly, infection of adult zebrafish with Pseudocapillaria tomentosa, a natural nematode pathogen of teleosts, caused marked increases in eosinophil number within the intestine. Together, these observations support a conserved role for eosinophils in the response to helminth antigens or infection and provide a new model to better understand how parasitic worms activate, co-opt, or evade the vertebrate immune response. |
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Authors:
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Keir M Balla; Geanncarlo Lugo-Villarino; Jan M Spitsbergen; David L Stachura; Yan Hu; Karina Bañuelos; Octavio Romo-Fewell; Raffi V Aroian; David Traver |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-08-16 |
Journal Detail:
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Title: Blood Volume: 116 ISSN: 1528-0020 ISO Abbreviation: Blood Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-12 Completed Date: 2010-12-16 Revised Date: 2011-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
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Languages: eng Pagination: 3944-54 Citation Subset: AIM; IM |
Affiliation:
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Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA 92093-0380, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified Antigens, Helminth Base Sequence Cell Degranulation / immunology DNA Primers / genetics Enoplida Infections / blood, immunology, parasitology Eosinophilia / etiology, immunology, parasitology Eosinophils / cytology, immunology, parasitology, physiology* GATA2 Transcription Factor / genetics, metabolism Host-Parasite Interactions Neutrophils / physiology Trichuroidea / immunology, pathogenicity Zebrafish / blood*, genetics, immunology, parasitology Zebrafish Proteins / genetics, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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3R01DK074482-01/DK/NIDDK NIH HHS; 5P30CA23100-22S2/CA/NCI NIH HHS; 5T34GM08303/GM/NIGMS NIH HHS; ES00210/ES/NIEHS NIH HHS; ES011587/ES/NIEHS NIH HHS; ES013124/ES/NIEHS NIH HHS; ES03850/ES/NIEHS NIH HHS; GMO68524//PHS HHS; R01-AI056189/AI/NIAID NIH HHS; R01-DK074482/DK/NIDDK NIH HHS; RR12546/RR/NCRR NIH HHS; T32-HL086344/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Helminth; 0/DNA Primers; 0/GATA2 Transcription Factor; 0/GATA2 protein, zebrafish; 0/Zebrafish Proteins |
| Comments/Corrections | |
Comment In:
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Blood. 2010 Nov 11;116(19):3692-3
[PMID:
21071614
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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