Document Detail


Enzymic synthesis of indole-3-acetyl-1-O-beta-d-glucose. II. Metabolic characteristics of the enzyme.
MedLine Citation:
PMID:  11537439     Owner:  NASA     Status:  MEDLINE    
Abstract/OtherAbstract:
The synthesis of indole-3-acetyl-1-O-beta-D-glucose from indole-3-acetic acid (IAA) and uridine diphosphoglucose (UDPG) has been shown to be a reversible reaction with the equilibrium away from ester formation and toward formation of IAA. The enzyme occurs primarily in the liquid endosperm of the corn kernel but some activity occurs in the embryo. It is relatively specific showing no glucose ester formation with oxindole-3-acetic acid or 7-hydroxy-oxindole-3-acetic acid, and low activity with phenylpropene acids, such as rho-coumaric acid. The enzyme is also specific for the nucleotide sugar showing no activity with UDPGalactose or UDPXylose. The enzyme is inhibited by inorganic pyrophosphate, by phosphate esters and by phospholipids, particularly phosphatidyl ethanolamine. The enzyme is inhibited by zeatin, by 2,4-dichlorophenoxy-acetic acid, by IAA-myo-inositol and IAA-glucan, but not by zeatin riboside, and only weakly by gibberellic acid, abscisic acid and kinetin. The reaction is slightly stimulated by both calcium and calmodulin and, in some cases, by thiol compounds. The role of this enzyme in the homeostatic control of indole-3-acetic acid levels in Zea mays is discussed.
Authors:
A J Leznicki; R S Bandurski
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Plant physiology     Volume:  88     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1988  
Date Detail:
Created Date:  1996-10-07     Completed Date:  1996-10-07     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1481-5     Citation Subset:  S    
Affiliation:
Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824-1312, USA.
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MeSH Terms
Descriptor/Qualifier:
2,4-Dichlorophenoxyacetic Acid / pharmacology
Chromatography / methods
Diphosphates / pharmacology
Glucans / pharmacology
Glucosyltransferases / antagonists & inhibitors,  metabolism*
Indoleacetic Acids / metabolism*
Phospholipids / pharmacology
Plant Growth Regulators / metabolism*,  pharmacology
Substrate Specificity
Uridine Diphosphate / metabolism
Uridine Diphosphate Glucose / metabolism*
Zea mays / enzymology*,  metabolism
Zeatin / pharmacology
Grant Support
ID/Acronym/Agency:
DMB-8504231/MB/BHP HRSA HHS
Chemical
Reg. No./Substance:
0/Diphosphates; 0/Glucans; 0/Indoleacetic Acids; 0/Phospholipids; 0/Plant Growth Regulators; 133-89-1/Uridine Diphosphate Glucose; 1637-39-4/Zeatin; 19817-95-9/1-O-indol-3-ylacetylglucose; 58-98-0/Uridine Diphosphate; 87-51-4/indoleacetic acid; 94-75-7/2,4-Dichlorophenoxyacetic Acid; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.121/indole-3-acetate beta-glucosyltransferase
Investigator
Investigator/Affiliation:
R S Bandurski / MI St U, East Lansing
Comments/Corrections

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