| Enzymic flow injection determination of free L-carnitine in infant formulae. | |
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MedLine Citation:
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PMID: 9474809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Infant formula samples were analysed to determine their free L-carnitine content by using flow injection (FI) with an incorporated immobilised carnitine acetyltransferase bioreactor. The methodology was based on the spectrophotometric determination through its reaction with carnitine acetyltransferase coupled with acetyl coenzyme A (acetyl-CoA) and dithiobenzoate. The merging zones technique was used to minimise acetyl CoA consumption. Linearity was observed over the concentration range 10-80 mg l-1 with L-carnitine as standard (r = 0.9998) and the rate of analysis was 50 h-1 infant formula samples. The enzymic FI method afforded a low RSD (2.2%). Comparisons were made with other methods of known accuracy. The enzymic reactors were stable for 3 months when used daily at the optimum pH. |
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Authors:
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I M Ferreira; M N Macedo; M A Ferreira |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Analyst Volume: 122 ISSN: 0003-2654 ISO Abbreviation: Analyst Publication Date: 1997 Dec |
Date Detail:
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Created Date: 1998-03-19 Completed Date: 1998-03-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1539-41 Citation Subset: IM |
Affiliation:
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CEQUP/Laboratório de Bromatologia, Faculdade de Farmácia, Universidade do Porto, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bioreactors Carnitine / analysis* Carnitine Acyltransferases Flow Injection Analysis Infant Food* |
| Chemical | |
Reg. No./Substance:
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541-15-1/Carnitine; EC 2.3.1.-/Carnitine Acyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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