Document Detail

Enzymic creatinine assay: a new colorimetric method based on hydrogen peroxide measurement.
MedLine Citation:
PMID:  6872208     Owner:  NLM     Status:  MEDLINE    
We describe a new colorimetric method for measuring creatinine in serum and urine. Creatinine hydrolysis is catalyzed by creatinine amidohydrolase, and the creatine so produced is assayed in reactions catalyzed sequentially by creatine amidinohydrolase and sarcosine oxidase in a system that generates hydrogen peroxide. The hydrogen peroxide is measured at 510 nm in a reaction catalyzed by horseradish peroxidase, with 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone as the chromogen. This series of reactions is complete in 30 min at room temperature. A blank sample measurement corrects for endogenous creatine. The standard curve is linear for creatinine concentrations as great as 2.21 mmol/L. Analytical recovery of creatinine in human sera and urine averaged 99.8%. Within-run and between-run precision studies gave CVs of less than or equal to 3.3 and less than or equal to 4.3% for a serum with a creatinine concentration of 69 mumol/L. Results by this method agree well (r greater than 0.99) with those by both the enzymic ultraviolet method of Wahlefeld and the fuller's earth/Jaffé method.
P Fossati; L Prencipe; G Berti
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  29     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1983 Aug 
Date Detail:
Created Date:  1983-09-09     Completed Date:  1983-09-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1494-6     Citation Subset:  IM    
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MeSH Terms
Chemical Phenomena
Creatinine / analysis*,  blood,  urine
Evaluation Studies as Topic
Hydrogen Peroxide / analysis*
Reg. No./Substance:
0/Benzenesulfonates; 26281-43-6/3,5-dichloro-2-hydroxybenzenesulfonic acid; 60-27-5/Creatinine; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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