| Enzymic creatinine assay: a new colorimetric method based on hydrogen peroxide measurement. | |
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MedLine Citation:
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PMID: 6872208 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We describe a new colorimetric method for measuring creatinine in serum and urine. Creatinine hydrolysis is catalyzed by creatinine amidohydrolase, and the creatine so produced is assayed in reactions catalyzed sequentially by creatine amidinohydrolase and sarcosine oxidase in a system that generates hydrogen peroxide. The hydrogen peroxide is measured at 510 nm in a reaction catalyzed by horseradish peroxidase, with 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone as the chromogen. This series of reactions is complete in 30 min at room temperature. A blank sample measurement corrects for endogenous creatine. The standard curve is linear for creatinine concentrations as great as 2.21 mmol/L. Analytical recovery of creatinine in human sera and urine averaged 99.8%. Within-run and between-run precision studies gave CVs of less than or equal to 3.3 and less than or equal to 4.3% for a serum with a creatinine concentration of 69 mumol/L. Results by this method agree well (r greater than 0.99) with those by both the enzymic ultraviolet method of Wahlefeld and the fuller's earth/Jaffé method. |
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Authors:
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P Fossati; L Prencipe; G Berti |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Clinical chemistry Volume: 29 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1983 Aug |
Date Detail:
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Created Date: 1983-09-09 Completed Date: 1983-09-09 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1494-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Benzenesulfonates Chemical Phenomena Chemistry Colorimetry Creatinine / analysis*, blood, urine Evaluation Studies as Topic Humans Hydrogen Peroxide / analysis* Methods Spectrophotometry |
| Chemical | |
Reg. No./Substance:
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0/Benzenesulfonates; 26281-43-6/3,5-dichloro-2-hydroxybenzenesulfonic acid; 60-27-5/Creatinine; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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