Document Detail


Enzymic creatinine assay: a new colorimetric method based on hydrogen peroxide measurement.
MedLine Citation:
PMID:  6872208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a new colorimetric method for measuring creatinine in serum and urine. Creatinine hydrolysis is catalyzed by creatinine amidohydrolase, and the creatine so produced is assayed in reactions catalyzed sequentially by creatine amidinohydrolase and sarcosine oxidase in a system that generates hydrogen peroxide. The hydrogen peroxide is measured at 510 nm in a reaction catalyzed by horseradish peroxidase, with 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone as the chromogen. This series of reactions is complete in 30 min at room temperature. A blank sample measurement corrects for endogenous creatine. The standard curve is linear for creatinine concentrations as great as 2.21 mmol/L. Analytical recovery of creatinine in human sera and urine averaged 99.8%. Within-run and between-run precision studies gave CVs of less than or equal to 3.3 and less than or equal to 4.3% for a serum with a creatinine concentration of 69 mumol/L. Results by this method agree well (r greater than 0.99) with those by both the enzymic ultraviolet method of Wahlefeld and the fuller's earth/Jaffé method.
Authors:
P Fossati; L Prencipe; G Berti
Related Documents :
7226538 - A highly sensitive colorimetric determination of serum copper using alpha, beta, gamma,...
11052688 - Determination of glucosinolates in canola seeds using anion exchange membrane extractio...
21298778 - Chiral hplc separation and absolute configuration assignment of a series of new triazol...
15533688 - Overestimation of rifampicin during colorimetric analysis of anti-tuberculosis products...
1117178 - The detection of carotenoid pigments in human skin.
18399438 - Removal of pesticides from white wine by the use of fining agents and filtration.
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  29     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1983 Aug 
Date Detail:
Created Date:  1983-09-09     Completed Date:  1983-09-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1494-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Benzenesulfonates
Chemical Phenomena
Chemistry
Colorimetry
Creatinine / analysis*,  blood,  urine
Evaluation Studies as Topic
Humans
Hydrogen Peroxide / analysis*
Methods
Spectrophotometry
Chemical
Reg. No./Substance:
0/Benzenesulfonates; 26281-43-6/3,5-dichloro-2-hydroxybenzenesulfonic acid; 60-27-5/Creatinine; 7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Improved turbidimetric assay for lysozyme in urine.
Next Document:  Interference by hemolysis with various methods for total calcium and its correction by trichloroacet...