| Enzymic mechanisms of starch breakdown in germinating rice seeds: 7. Amylase formation in the epithelium. | |
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MedLine Citation:
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PMID: 16660724 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
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The time sequence analysis of the starch digestion pattern of the thin sectioned germinating rice (Oryza sativa L.) seed specimens using the starch film method showed that at the initial stage amylase activity was almost exclusively localized in the epithelium septum between the scutellum and endosperm. Starch breakdown in the endosperm tissues began afterward; amylase activity in the aleurone layers was detectable only after 2 days. Polyacrylamide gel electrofocusing (pH 4 to 6) revealed nearly the same zymogram patterns between endosperm and scutellum extracts, although additional amylase bands appeared in the endosperm extracts at later germination stages (4 to 6 days). These are presumably attributable to the newly synthesized enzyme molecules in the aleurone cells. |
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Authors:
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K Okamoto; T Akazawa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Plant physiology Volume: 63 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 1979 Feb |
Date Detail:
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Created Date: 2010-06-29 Completed Date: 2010-06-29 Revised Date: 2010-09-15 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 336-40 Citation Subset: - |
Affiliation:
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Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University, Chikusa, Nagoya 464 Japan. |
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