Document Detail


Enzymes that scavenge reactive oxygen species are down-regulated prior to gibberellic acid-induced programmed cell death in barley aleurone.
MedLine Citation:
PMID:  11351079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gibberellins (GAs) initiate a series of events that culminate in programmed cell death, whereas abscisic acid (ABA) prevents this process. Reactive oxygen species (ROS) are key elements in aleurone programmed cell death. Incubation of barley (Hordeum vulgare) aleurone layers in H2O2 causes rapid death of all cells in GA- but not ABA-treated layers. Sensitivity to H2O2 in GA-treated aleurone cells results from a decreased ability to metabolize ROS. The amounts and activities of ROS scavenging enzymes, including catalase (CAT), ascorbate peroxidase, and superoxide dismutase are strongly down-regulated in aleurone layers treated with GA. CAT activity, protein, and Cat2 mRNA decline rapidly following exposure of aleurone layers to GA. In ABA-treated layers, on the other hand, the amount and activity of CAT and Cat2 mRNA increases. Incubation in ABA maintains high amounts of ascorbate peroxidase and superoxide dismutase, whereas GA brings about a rapid reduction in the amounts of these enzymes. These data imply that GA-treated cells loose their ability to scavenge ROS and that this loss ultimately results in oxidative damage and cell death. ABA-treated cells, on the other hand, maintain their ability to scavenge ROS and remain viable.
Authors:
A Fath; P C Bethke; R L Jones
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Plant physiology     Volume:  126     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-14     Completed Date:  2001-07-26     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  156-66     Citation Subset:  IM    
Affiliation:
Department of Plant and Microbial Biology, University of California, Berkeley, California 94720-3102, USA afath@nature.berkeley.edu.
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MeSH Terms
Descriptor/Qualifier:
Abscisic Acid / pharmacology
Apoptosis / drug effects
Catalase / genetics,  metabolism*
Down-Regulation*
Gibberellins / pharmacology*
Hordeum / cytology,  enzymology*,  metabolism
Peroxidases / metabolism*
Reactive Oxygen Species*
Superoxide Dismutase / metabolism*
Chemical
Reg. No./Substance:
0/Gibberellins; 0/Reactive Oxygen Species; 21293-29-8/Abscisic Acid; 77-06-5/gibberellic acid; EC 1.11.1.-/Peroxidases; EC 1.11.1.11/ascorbate peroxidase; EC 1.11.1.6/Catalase; EC 1.15.1.1/Superoxide Dismutase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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