Document Detail

Enzymes for biosynthesis de novo and elongation of fatty acids in mycobacteria grown in host cells: is Mycobacterium leprae competent in fatty acid biosynthesis?
MedLine Citation:
PMID:  2191079     Owner:  NLM     Status:  MEDLINE    
Fatty acid synthetase activity in extracts of Mycobacterium leprae was equivalent to 1.7 pmol malonyl-CoA incorporated into fatty acid min-1 (mg protein)-1. This activity--if representative of living M. leprae organisms--is insufficient to enable them to synthesize their lipid requirements rapidly enough to support growth. The major activity for scavenging fatty acids in extracts of Mycobacterium microti and Mycobacterium avium, as well as in extracts of M. leprae, was acetyl-CoA-dependent fatty acyl-CoA 'elongase'. This activity was about four times higher in M. avium and M. microti grown in a medium which contained lipids, or when grown in mice, than in medium without added lipids. In contrast, the de novo fatty acid synthetase activity was repressed in M. avium and M. microti when grown in medium that contained lipids, or when grown in mice. These results are consistent with the hypothesis that mycobacteria grown in vivo preferentially scavenge lipids from the host cells, and suggest that a source of lipid should be included in media for attempted axenic isolation of M. leprae.
P R Wheeler; K Bulmer; C Ratledge
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of general microbiology     Volume:  136     ISSN:  0022-1287     ISO Abbreviation:  J. Gen. Microbiol.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-07-13     Completed Date:  1990-07-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375371     Medline TA:  J Gen Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  211-7     Citation Subset:  IM    
Department of Applied Biology, University of Hull, UK.
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MeSH Terms
Acetyltransferases / metabolism
Fatty Acid Synthetase Complex / metabolism*
Fatty Acids / metabolism*
Malonyl Coenzyme A / physiology
Mycobacterium / enzymology,  metabolism*
Mycobacterium avium / enzymology,  metabolism
Mycobacterium leprae / enzymology,  metabolism
Reg. No./Substance:
0/Fatty Acids; 524-14-1/Malonyl Coenzyme A; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases; EC 6.-/Fatty Acid Synthetase Complex

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