Document Detail

Enzyme variation among clones of Trypanosoma cruzi.
MedLine Citation:
PMID:  6219197     Owner:  NLM     Status:  MEDLINE    
The genetic relationships within and between stocks of Trypanosoma cruzi were studied by the in vitro isolation of clones and sub-clones and by the comparison of their isoenzyme patterns in thin-layer starch-gel electrophoresis. Altogether 13 clones and 36 sub-clones were isolated from stocks CL89 and Y207 of T. cruzi. Employing the enzymes L-alanine aminotransferase (ALAT), L-aspartate aminotransferase (ASAT), glucose phosphate isomerase (GPI), malic enzyme (ME), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), and phosphoglucomutase (PGM), two zymodemes, B and C, emerged among the clones with distinct banding patterns. These zymodemes were consistently distinguished by ALAT, ASAT, GPI, G6PD, 6PGD, and PGM and the differences in enzyme profiles were simultaneously reflected in all six enzyme systems. That the enzymic characters as genetic determinants are stable was demonstrated after recloning and successive replica-platings, i.e., the distinct enzyme patterns remained consistent and homogeneous, the siblings retained the enzyme profiles of their parental clones, and no segregation of the enzyme patterns was observed. Our data from clone and sub-clone examinations show that the isoenzymes act as labels to characterize T. cruzi stocks. Furthermore, enzyme variation was demonstrated among clones isolated from stock CL89.
S S Goldberg; A A Silva Perelra
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of parasitology     Volume:  69     ISSN:  0022-3395     ISO Abbreviation:  J. Parasitol.     Publication Date:  1983 Feb 
Date Detail:
Created Date:  1983-04-21     Completed Date:  1983-04-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7803124     Medline TA:  J Parasitol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  91-6     Citation Subset:  IM    
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MeSH Terms
Alanine Transaminase / analysis
Aspartate Aminotransferases / analysis
Electrophoresis, Starch Gel
Glucose-6-Phosphate Isomerase / analysis
Glucosephosphate Dehydrogenase / analysis
Isoenzymes / analysis*
Phosphoglucomutase / analysis
Phosphogluconate Dehydrogenase / analysis
Trypanosoma cruzi / enzymology*,  genetics
Reg. No./Substance:
0/Isoenzymes; EC Dehydrogenase; EC Dehydrogenase; EC Aminotransferases; EC Transaminase; EC Isomerase; EC

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