Document Detail


Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix.
MedLine Citation:
PMID:  17971995     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The synthesis of a Ni-nitrilotriacetic acid (Ni-NTA) attached via a new tyrosine-based linker matrix on monolithic crosslinked poly(vinyl benzyl chloride)/poly(vinylpyrrolidinone) is described. This matrix is incorporated inside a microstructured PASSflow reactor which was used for automatic purification and immobilisation of His(6)-tagged proteins. These could be used as stable and highly active biocatalysts for the synthesis of (R)-benzoin (6), (R)-2-hydroxy-1-phenylpropan-1-one (7) and 6-O-acetyl-D-glucal (17) in a flow-through mode.
Authors:
Gerald Dräger; Csilla Kiss; Ulrich Kunz; Andreas Kirschning
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-05
Journal Detail:
Title:  Organic & biomolecular chemistry     Volume:  5     ISSN:  1477-0520     ISO Abbreviation:  Org. Biomol. Chem.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-31     Completed Date:  2008-01-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101154995     Medline TA:  Org Biomol Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  3657-64     Citation Subset:  IM    
Affiliation:
Institut für Organische Chemie and Zentrum für Biomolekulare Wirkstoffchemie (BMWZ), Leibniz Universität Hannover, Schneiderberg 1b, Hannover, Germany. draeger@oci.uni-hannover.de
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MeSH Terms
Descriptor/Qualifier:
Aldehyde-Lyases / isolation & purification*
Benzoin / chemistry,  metabolism
Bioreactors*
Catalysis
Enzymes, Immobilized / metabolism
Escherichia coli / enzymology*
Esters / metabolism
Hydrolysis
Lysine / metabolism
Nickel / metabolism*
Nitrilotriacetic Acid / metabolism*
Povidone / metabolism*
Stereoisomerism
Tyrosine / metabolism*
Chemical
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Esters; 119-53-9/Benzoin; 139-13-9/Nitrilotriacetic Acid; 55520-40-6/Tyrosine; 56-87-1/Lysine; 7440-02-0/Nickel; 9003-39-8/Povidone; EC 4.1.2.-/Aldehyde-Lyases; EC 4.1.2.-/benzaldehyde lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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