| Enzyme-purification and catalytic transformations in a microstructured PASSflow reactor using a new tyrosine-based Ni-NTA linker system attached to a polyvinylpyrrolidinone-based matrix. | |
| | |
MedLine Citation:
|
PMID: 17971995 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The synthesis of a Ni-nitrilotriacetic acid (Ni-NTA) attached via a new tyrosine-based linker matrix on monolithic crosslinked poly(vinyl benzyl chloride)/poly(vinylpyrrolidinone) is described. This matrix is incorporated inside a microstructured PASSflow reactor which was used for automatic purification and immobilisation of His(6)-tagged proteins. These could be used as stable and highly active biocatalysts for the synthesis of (R)-benzoin (6), (R)-2-hydroxy-1-phenylpropan-1-one (7) and 6-O-acetyl-D-glucal (17) in a flow-through mode. |
| | |
Authors:
|
Gerald Dräger; Csilla Kiss; Ulrich Kunz; Andreas Kirschning |
Related Documents
:
|
10092385 - Surface electrical properties of barium sulfate modified by adsorption of poly alpha, b... 20674365 - Nanostructured bacterial materials for innovative medicines. 14653285 - The effect of antimicrobial pre-treatments on the performance of resin composite restor... 3860145 - Synthetic polymers as drugs. 20715835 - Kinetic resolution of racemic 2,3-allenoates by organocatalytic asymmetric 1,3-dipolar ... 1618845 - Partial characterization of the major lipooligosaccharide from a strain of haemophilus ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-05 |
Journal Detail:
|
Title: Organic & biomolecular chemistry Volume: 5 ISSN: 1477-0520 ISO Abbreviation: Org. Biomol. Chem. Publication Date: 2007 Nov |
Date Detail:
|
Created Date: 2007-10-31 Completed Date: 2008-01-08 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101154995 Medline TA: Org Biomol Chem Country: England |
Other Details:
|
Languages: eng Pagination: 3657-64 Citation Subset: IM |
Affiliation:
|
Institut für Organische Chemie and Zentrum für Biomolekulare Wirkstoffchemie (BMWZ), Leibniz Universität Hannover, Schneiderberg 1b, Hannover, Germany. draeger@oci.uni-hannover.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Aldehyde-Lyases
/
isolation & purification* Benzoin / chemistry, metabolism Bioreactors* Catalysis Enzymes, Immobilized / metabolism Escherichia coli / enzymology* Esters / metabolism Hydrolysis Lysine / metabolism Nickel / metabolism* Nitrilotriacetic Acid / metabolism* Povidone / metabolism* Stereoisomerism Tyrosine / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Enzymes, Immobilized; 0/Esters; 119-53-9/Benzoin; 139-13-9/Nitrilotriacetic Acid; 55520-40-6/Tyrosine; 56-87-1/Lysine; 7440-02-0/Nickel; 9003-39-8/Povidone; EC 4.1.2.-/Aldehyde-Lyases; EC 4.1.2.-/benzaldehyde lyase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Synthesis, characterisation and anti-protozoal activity of carbamate-derived polyazamacrocycles.
Next Document: Benzimidazole- and benzothiazole-quinones: excellent substrates for NAD(P)H:quinone oxidoreductase 1...