Document Detail


Enzyme-linked immunosorbent assay for measurement of allergen-specific IgE antibodies in canine serum.
MedLine Citation:
PMID:  2619113     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A micro-ELISA, using horseradish peroxidase-conjugated anti-canine IgE and polystyrene microtitration wells for detection of allergen-specific IgE in canine serum, was developed. Specificity of anti-canine IgE was confirmed by reversed cutaneous anaphylaxis evaluations, gel-precipitation reactions, immunoelectrophoresis, immunoaffinity chromatography, and heat inactivation. Individual allergen blanks were used to account for variable nonspecific binding among various allergens, and results were normalized using 4 reference sera. Coefficients of variation for intra-assay and interassay variability ranged from 0.77 to 5.66% and 3.15 to 9.83%, respectively. Results observed with wells coated with mixtures of various allergen extracts yielded results approximately equal to results (average) of wells containing individual components. Agreement between ELISA and skin test results ranged from 43 to 64%, depending on allergen used.
Authors:
M L Kleinbeck; M J Hites; J L Loker; R E Halliwell; K W Lee
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of veterinary research     Volume:  50     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1990-03-06     Completed Date:  1990-03-06     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1831-9     Citation Subset:  IM    
Affiliation:
Research and Development Department, Iatric Corporation, Tempe, AZ 85282.
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MeSH Terms
Descriptor/Qualifier:
Allergens / immunology*
Animals
Dog Diseases / diagnosis*
Dogs
Enzyme-Linked Immunosorbent Assay*
Female
Hypersensitivity, Immediate / diagnosis,  veterinary*
Immunoglobulin E / analysis*
Intradermal Tests / veterinary
Male
Predictive Value of Tests
Reproducibility of Results
Chemical
Reg. No./Substance:
0/Allergens; 37341-29-0/Immunoglobulin E

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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