| Enzyme-linked immunosorbent assay for determination of antibodies to xanthine oxidase. | |
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MedLine Citation:
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PMID: 2377033 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An enzyme-linked immunosorbent assay (ELISA) was developed to detect IgG, IgA and IgM antibodies to xanthine oxidase. The method used xanthine oxidase to coat sample wells on microtitre plates. The anti-xanthine oxidase concentrations were determined by reference to standard curves constructed by coating plates with anti-IgG, anti-IgA and anti-IgM to capture antibodies of different classes in standard human serum. The standard curves for IgG, IgA and IgM had a working range of 0 to about 60 ng/ml, and all results with commercial quality control serum fell within expected ranges. The coefficients of variation (CV) for within-batch precision (n = 30) and between-batch precision (n = 20) for IgG and IgM were about 9% and 12% respectively. The detection limit was 2 ng/ml. The ELISA was applied to assay serum samples of 110 Chinese and 110 European healthy subjects. A positively-skewed distribution in their anti-xanthine oxidase IgG and IgM levels was observed. |
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Authors:
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Y L Ng; W H Lewis; S H Chui |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Medical laboratory sciences Volume: 47 ISSN: 0308-3616 ISO Abbreviation: Med Lab Sci Publication Date: 1990 Jan |
Date Detail:
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Created Date: 1990-09-04 Completed Date: 1990-09-04 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7609161 Medline TA: Med Lab Sci Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 30-5 Citation Subset: IM |
Affiliation:
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Department of Health Sciences, Hong Kong Polytechnic, Hung Hom, Kowloon. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies
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analysis* Enzyme-Linked Immunosorbent Assay / methods* Humans Immunoglobulin A / immunology Immunoglobulin G / immunology Immunoglobulin M / immunology Xanthine Oxidase / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Immunoglobulin A; 0/Immunoglobulin G; 0/Immunoglobulin M; EC 1.17.3.2/Xanthine Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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