Document Detail


Enzyme-linked immunosorbent assay for detection of staphylococcal enterotoxins in foods.
MedLine Citation:
PMID:  7159082     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An enzyme-linked immunosorbent assay (ELISA) was developed for detection of staphylococcal enterotoxins in foods. The "double-antibody sandwich" protocol combines parts of several procedures reported previously. Horseradish peroxidase was conjugated to antibody specific for an enterotoxin, and the antibody-enzyme conjugate was assayed with a 2,2'-azino-di-(3-ethylbenzthiazoline sulfonic acid)-H2O2 substrate solution. Enterotoxins were added to a variety of foods that were representative of those implicated in staphylococcal food poisoning outbreaks. Extracts of the foods were assayed by the ELISA and radioimmunoassay. Enterotoxin levels below 1 ng/g of food were consistently detectable by the ELISA. These results compared favorably with those of the radioimmunoassay. Experiments confirmed the interference of protein A in double-antibody sandwich ELISAs. Although protein A interference has not been demonstrated to be a problem in food extracts, we suggest a screen for protein A interference in which immunoglobulin G from nonimmunized rabbits is used. All of the known staphylococcal enterotoxins could be detected by this method. Analysis of a food product for entertoxin by the ELISA can be completed in an 8-h working day.
Authors:
R C Freed; M L Evenson; R F Reiser; M S Bergdoll
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  44     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1982 Dec 
Date Detail:
Created Date:  1983-03-11     Completed Date:  1983-03-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1349-55     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Enterotoxins / analysis*
Enzyme-Linked Immunosorbent Assay
Evaluation Studies as Topic
Food Contamination*
Meat
Radioimmunoassay
Staphylococcal Protein A
Staphylococcus aureus*
Swine
Grant Support
ID/Acronym/Agency:
EF 07015//PHS HHS
Chemical
Reg. No./Substance:
0/Enterotoxins; 0/Staphylococcal Protein A; 37337-57-8/enterotoxin A, Staphylococcal
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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