Document Detail

Enzyme-linked immunosorbent assay-based inhibition test for neutralizing antibodies to polioviruses as an alternative to the neutralization test in tissue culture.
MedLine Citation:
PMID:  8576347     Owner:  NLM     Status:  MEDLINE    
A poliovirus-binding inhibition test (PoBI test) was established for the quantitative determination of antibodies to polioviruses and was evaluated in comparison with the conventional neutralization test (NT). The first step of the PoBI test is an incubation of serial dilutions of test samples with inactivated poliovirus followed by the detection of free viral epitopes by a double antibody sandwich enzyme-linked immunosorbent assay with type-specific capture polyclonal antisera and type-specific neutralizing monoclonal indicator antibodies. A comparison of the PoBI test with the conventional NT for antibodies to all three types in 100 human serum samples showed excellent correlations (r > 0.95) over a wide range of antibody concentrations. The PoBI test, not necessitating live virus and tissue culture facilities, could be a simple alternative to the NT, and the principle of the assay is potentially applicable to other microbial systems.
G Edevåg; B Wahren; A D Osterhaus; V A Sundqvist; M Granström
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  33     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1996-03-12     Completed Date:  1996-03-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2927-30     Citation Subset:  IM    
Department of Virology, Swedish Institute for Infectious Disease Control, Stockholm University School of Health and Science, Sweden.
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MeSH Terms
Antibodies, Viral / blood*
Antigen-Antibody Reactions
Antigens, Viral / diagnostic use
Enzyme-Linked Immunosorbent Assay / methods*
Guinea Pigs
Immunologic Techniques
Immunologic Tests
Neutralization Tests / methods*
Poliovirus / immunology*
Sensitivity and Specificity
Reg. No./Substance:
0/Antibodies, Viral; 0/Antigens, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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