Document Detail


Enzyme immunoassay for semicarbazide--the nitrofuran metabolite and food contaminant.
MedLine Citation:
PMID:  17499072     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Semicarbazide (SEM), the marker residue for the banned nitrofuran veterinary antibiotic nitrofurazone (NFZ), has been detected regularly in foods (47% of recent nitrofuran EU Rapid Alerts involve SEM). However, the validity of SEM as a definitive marker for NFZ has been undermined by SEM arising from other sources including azodicarbonamide, a plastics blowing agent and flour treatment additive. An inexpensive screening test for SEM in food matrices is needed--all SEM testing currently uses expensive LC-MS/MS instrumentation. We now report the first production of antibodies against derivatised SEM. A novel carboxyphenyl SEM derivative was used to raise a polyclonal antibody that has been incorporated into a semi-quantitative microtitre plate ELISA, validated according to the criteria set out in Commission Decision 2002/657/EC, for use with chicken muscle. The antibody is highly specific for derivatised SEM, cross-reactivity being 1.7% with NFZ and negligible with a wide range of other nitrofurans and poultry drugs. Samples are derivatised with o-nitrobenzaldehyde and simultaneously protease digested before extraction by cation exchange SPE. The ELISA has a SEM detection capability (CCbeta) of 0.25 microg kg(-1) when a threshold of 0.21 microg kg(-1) is applied to the selection of samples for confirmation (lowest observed 0.25 microg kg(-1) fortified sample, n=20), thus satisfying the EU nitrofurans' minimum required performance limit of 1 microg kg(-1). NFZ-incurred muscles (12) containing SEM at 0.5-5.0 microg kg(-1) by LC-MS/MS, all screened positive by this ELISA protocol which is also applicable to egg and chicken liver.
Authors:
Kevin M Cooper; Jeanne V Samsonova; Laura Plumpton; Christopher T Elliott; D Glenn Kennedy
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-04-19
Journal Detail:
Title:  Analytica chimica acta     Volume:  592     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-05-14     Completed Date:  2007-05-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  64-71     Citation Subset:  IM    
Affiliation:
Queen's University Belfast, Department of Veterinary Science, Northern Ireland, UK.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, Liquid
Enzyme-Linked Immunosorbent Assay / methods*
Food Contamination*
Nitrofurans / metabolism*
Reproducibility of Results
Semicarbazides / analysis*
Sensitivity and Specificity
Tandem Mass Spectrometry
Chemical
Reg. No./Substance:
0/Nitrofurans; 0/Semicarbazides; 57-56-7/carbamylhydrazine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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