Document Detail


Enzyme immunoassay for determination of peanut proteins in food products.
MedLine Citation:
PMID:  8946719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Food allergy presents a problem for many parts of society, including sensitive subjects, schools, health authorities, and the food industry. Once food allergy is diagnosed, dietary avoidance is the principle method of management. Because trace levels of peanuts can elicit an adverse to fatal reaction, unintentional exposure to the offending allergens may have devastating consequences to sensitive individuals. However, determination of trace amounts of unintentional peanut contamination in our food supply is very difficult. Recently, we developed polyclonal antibodies specific to peanut proteins that do not cross-react with 22 legumes, tree nuts, or other common snack ingredients. An antiserum containing the polyclonal antibodies was used to develop a sensitive enzyme-linked immunosorbent assay (ELISA) for determination of peanut proteins in snack foods. This study reports the first successful ELISA test to detect trace amounts of peanut allergens in a variety of foods. The concentration of peanut protein that inhibited 50% of antibody-antigen binding (IC50) was 12 ng/mL, the linear range was 1-63 ng/mL, and the detection limit was 400 ng/g (ppb) for the various foods tested. Recoveries ranged from 68 to 90%, with coefficients of variation of 2-22%, depending on the commodity. Using this new procedure, allergy-related complaint samples from various food groups were analyzed, and undeclared peanut proteins were identified in some products.
Authors:
J M Yeung; P G Collins
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  79     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    1996 Nov-Dec
Date Detail:
Created Date:  1997-01-16     Completed Date:  1997-01-16     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1411-6     Citation Subset:  IM    
Affiliation:
Health Protection Branch, Food Directorate, Bureau of Chemical Safety, Food Research Division, Ottawa, ON, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibody Formation
Antigen-Antibody Complex
Arachis hypogaea / chemistry*
Buffers
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Food Analysis*
Food Hypersensitivity / diagnosis,  etiology
Humans
Immunization
Lethal Dose 50
Male
Plant Proteins / analysis*
Rabbits
Serum Albumin / metabolism
Chemical
Reg. No./Substance:
0/Antigen-Antibody Complex; 0/Buffers; 0/Plant Proteins; 0/Serum Albumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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