Document Detail


Enzyme histochemical analysis of lymphatic vessels in colon carcinoma: occurrence of lymphangiogenesis within the tumor.
MedLine Citation:
PMID:  16001629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND/AIMS: While it is generally recognized that the lymph vessel is absent in solid carcinoma, a few papers have reported the presence of intratumoral lymph vessels. The present study was carried out to clarify whether or not intratumoral lymphangiogenesis occurs. METHODOLOGY: To identify lymphatics in colon carcinomas, tumor-adjoining and normal submucosa, we tried using an enzymatic histochemistry procedure to examine the specific high activity of 5'-nucleotidase that is seen in lymphatic endothelial cells. Additionally, arterioles and venules were identified by alkaline phosphatase and dipeptidyl (amino) peptidase IV staining, respectively. RESULTS: Intratumoral lymphatic vessels were observed in 30 of 34 colon carcinomas (91%), and arterioles were found in all cases. However, no venules were identified within the tumor. The lymphatic density (mean +/- SD vessels/mm2) increased in the order of the submucosa near the tumor (21.9 +/- 9.5), normal submucosa (27.9 +/- 13.8) and tumor tissue (33.9 +/- 25.1). Intratumoral lymphatic density was significantly higher than that in the submucosa near the tumor (P<0.05). Intratumoral lymphatic density is related to arteriolar density (r=0.284, P=0.0012). The ratio of lymphatic/arteriolar density in the tumor was significantly higher than that seen in the submucosa near the tumor (0.78 +/- 0.76 vs. 0.51 +/- 0.36, P<0.05). Intratumoral lymphatic density was relatively higher in cases with lymph node metastasis than in those without metastasis, but was not related to tumor size, depth of tumor invasion, distant metastasis and TNM stage. CONCLUSIONS: Enzyme histochemistry revealed active lymphangiogenesis and the absence of venules within the tumor. Intratumoral lymphatic density was relatively correlated to arteriolar density. Enzyme histochemistry is a simple and quickly processed method that can be used to differentiate lymphatics from arterioles and venules.
Authors:
Shinichi Kuroyama; Nobuyuki Kobayashi; Makoto Ohbu; Yoshimasa Ohtani; Isao Okayasu; Akira Kakita
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Hepato-gastroenterology     Volume:  52     ISSN:  0172-6390     ISO Abbreviation:  Hepatogastroenterology     Publication Date:    2005 Jul-Aug
Date Detail:
Created Date:  2005-07-08     Completed Date:  2005-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8007849     Medline TA:  Hepatogastroenterology     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  1057-61     Citation Subset:  IM    
Affiliation:
Department of Surgery, Kitasato University School of Medicine, Sagamihara, Japan. s-kuro@kitasato-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
5'-Nucleotidase / metabolism*
Carcinoma / blood supply,  enzymology,  pathology*
Colonic Neoplasms / blood supply,  enzymology,  pathology*
Humans
Intestinal Mucosa / enzymology,  pathology
Lymphangiogenesis / physiology*
Lymphatic Vessels / enzymology*
Neoplasm Invasiveness
Neoplasm Staging
Neovascularization, Pathologic / pathology
Chemical
Reg. No./Substance:
EC 3.1.3.5/5'-Nucleotidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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