Document Detail


Enzyme electrochemical preparation of a 3-keto derivative of 1,5-anhydro-D-glucitol using glucose-3-dehydrogenase.
MedLine Citation:
PMID:  10849848     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel enzymatic organic synthesis was reported, utilizing glucose-3-dehydrogenase (G3DH) and its regeneration via electrochemical methods. We combined the water-soluble G3DH prepared from a marine bacterium, Halomonas sp. alpha-15, and electron mediator with the electrode system in order to regenerate the enzyme. Using this system, the conversion of 1,5-anhydro-D-glucitol (1,5AG), a diabetes marker in human blood, was investigated. The final yield of the product, 3-keto anhydroglucitol (3-ketoAG), which was identified by 13C nuclear magnetic resonance, was 82% based on the initial amount of 1,5AG. The electrochemical yield of the reaction proceeded almost stoichiometrically. The electrochemical conversion rate of 1,5AG was 1.24 mmol/(L.h), and the electrochemical yield of 1,5AG consumption was 80%, whereas that for 3-ketoAG was 60%.
Authors:
K Sode; H Sugiura; W Tsugawa; Y Watazu; T Hamafuji
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  84-86     ISSN:  0273-2289     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2000  
Date Detail:
Created Date:  2000-09-26     Completed Date:  2000-09-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  947-54     Citation Subset:  IM    
Affiliation:
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan. sode@cc.tuat.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Biological Markers / blood
Deoxyglucose* / blood
Diabetes Mellitus / blood,  diagnosis
Electrochemistry / methods
Glucose 1-Dehydrogenase
Glucose Dehydrogenases* / isolation & purification,  metabolism
Halomonas / enzymology
Humans
Molecular Structure
Seawater / microbiology
Sorbitol / analogs & derivatives,  chemical synthesis*,  chemistry
Chemical
Reg. No./Substance:
0/Biological Markers; 154-17-6/Deoxyglucose; 154-58-5/1,5-anhydroglucitol; 50-70-4/Sorbitol; EC 1.1.1.-/Glucose Dehydrogenases; EC 1.1.1.47/Glucose 1-Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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