| Enzyme electrochemical preparation of a 3-keto derivative of 1,5-anhydro-D-glucitol using glucose-3-dehydrogenase. | |
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MedLine Citation:
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PMID: 10849848 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel enzymatic organic synthesis was reported, utilizing glucose-3-dehydrogenase (G3DH) and its regeneration via electrochemical methods. We combined the water-soluble G3DH prepared from a marine bacterium, Halomonas sp. alpha-15, and electron mediator with the electrode system in order to regenerate the enzyme. Using this system, the conversion of 1,5-anhydro-D-glucitol (1,5AG), a diabetes marker in human blood, was investigated. The final yield of the product, 3-keto anhydroglucitol (3-ketoAG), which was identified by 13C nuclear magnetic resonance, was 82% based on the initial amount of 1,5AG. The electrochemical yield of the reaction proceeded almost stoichiometrically. The electrochemical conversion rate of 1,5AG was 1.24 mmol/(L.h), and the electrochemical yield of 1,5AG consumption was 80%, whereas that for 3-ketoAG was 60%. |
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Authors:
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K Sode; H Sugiura; W Tsugawa; Y Watazu; T Hamafuji |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 84-86 ISSN: 0273-2289 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2000 |
Date Detail:
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Created Date: 2000-09-26 Completed Date: 2000-09-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 947-54 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan. sode@cc.tuat.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biological Markers
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blood Deoxyglucose* / blood Diabetes Mellitus / blood, diagnosis Electrochemistry / methods Glucose 1-Dehydrogenase Glucose Dehydrogenases* / isolation & purification, metabolism Halomonas / enzymology Humans Molecular Structure Seawater / microbiology Sorbitol / analogs & derivatives, chemical synthesis*, chemistry |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 154-17-6/Deoxyglucose; 154-58-5/1,5-anhydroglucitol; 50-70-4/Sorbitol; EC 1.1.1.-/Glucose Dehydrogenases; EC 1.1.1.47/Glucose 1-Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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