| Enzyme-controlled scavenging of ascorbyl and 2,6-dimethoxy-semiquinone free radicals in Ehrlich ascites tumor cells. | |
| | |
MedLine Citation:
|
PMID: 3856273 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The rate of scavenging by Ehrlich ascites cells of anionic ascorbyl and 2,6-dimethoxy-p-semiquinone free radicals has been investigated by electron spin resonance spectroscopy both for viable cells and for subcellular fractions obtained by differential centrifugation. The scavenging activity is concluded to be associated with an NAD(P)H enzyme containing an active sulfhydryl group. Attempts to identify the enzyme with the reported properties of either semi-dehydro-ascorbate reductase or DT-diaphorase have not been successful. The overall free-radical scavenging activity for viable cells is dextrose dependent and is controlled by the coulombic barrier associated with the cell-surface charge. The cytotoxicity of the mixture of ascorbic acid with 2,6-dimethoxy-p-benzoquinone is concluded to result from a loss of NAD(P)H reducing power in the cells. |
| | |
Authors:
|
R Pethig; P R Gascoyne; J A McLaughlin; A Szent-Györgyi |
Related Documents
:
|
4374963 - A tumour cell aggregation promoting substance from rat ascites hepatoma cells. 3208913 - Restricted amino acid nutrition induces attachment to glass and spreading of ehrlich as... 15362883 - A three-dimensional flow control concept for single-cell experiments on a microchip. 1.... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 82 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1985 Mar |
Date Detail:
|
Created Date: 1985-04-08 Completed Date: 1985-04-08 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 1439-42 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Ascorbic Acid / metabolism* Benzoquinones* Carcinoma, Ehrlich Tumor / physiopathology* Female Free Radicals* Magnetic Resonance Spectroscopy Mice NAD / metabolism Quinones / metabolism* Sulfhydryl Compounds / metabolism Surface Properties |
| Chemical | |
Reg. No./Substance:
|
0/Benzoquinones; 0/Free Radicals; 0/Quinones; 0/Sulfhydryl Compounds; 26547-64-8/2,6-dimethoxysemiquinone radicals; 50-81-7/Ascorbic Acid; 53-84-9/NAD |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: On the origin of species of fibronectin.
Next Document: Calcium channel antagonists inhibit the acrosome reaction and bind to plasma membranes of sea urchin...