| Enzyme-assisted acidolysis of borage (Borago officinalis L.) and evening primrose (Oenothera biennis L.) oils: incorporation of omega-3 polyunsaturated fatty acids. | |
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MedLine Citation:
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PMID: 10552616 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lipase-catalyzed acidolysis of borage (Borago officinalis L.) and evening primrose (Oenothera biennisL.) oils with long-chain omega3 polyunsaturated fatty acids (PUFA), namely, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, was carried out in hexane, and the products were analyzed using gas chromatography. The most effective lipase for incorporation of omega3 PUFA into these oils was Pseudomonas sp. as compared to lipases from Mucor miehei and Candida antarctica. Response surface methodology was used to obtain a maximum yield of EPA+DHA incorporation while using the minimum amount of enzyme possible. The process variables studied were the amount of enzyme (150-350 units), reaction temperature (30-60 degrees C), and reaction time (6-30 h). All experiments were carried out according to a face-centered cube design. Under optimum conditions, incorporation of EPA+DHA was 35.5% in borage oil and 33. 6% in evening primrose oil. The modified borage and evening primrose oils containing gamma-linolenic acid, EPA, and DHA were successfully produced and may have potential health benefits. |
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Authors:
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S P Senanayake; F Shahidi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 47 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 1999 Aug |
Date Detail:
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Created Date: 2000-09-12 Completed Date: 2000-09-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3105-12 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Memorial University of Newfoundland, St. John's, Canada. fshahidi@morgan.ucs.mun.ca |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Catalysis Dietary Fats, Unsaturated* Docosahexaenoic Acids / chemistry Eicosapentaenoic Acid / chemistry Fatty Acids, Essential / chemistry* Fatty Acids, Omega-3 / chemistry* Linoleic Acids Lipase* Plant Oils / chemistry* gamma-Linolenic Acid |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats, Unsaturated; 0/Fatty Acids, Essential; 0/Fatty Acids, Omega-3; 0/Linoleic Acids; 0/Plant Oils; 0/borage oil; 1553-41-9/Eicosapentaenoic Acid; 25167-62-8/Docosahexaenoic Acids; 506-26-3/gamma-Linolenic Acid; 65546-85-2/Efamol; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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