Document Detail


Enzyme assay for identification of pectin and pectin derivatives, based on recombinant pectate lyase.
MedLine Citation:
PMID:  11767155     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple method was developed for fast identification of pectin, based on a recombinant endopectate lyase cloned from Aspergillus niger. When pectin was demethylated and treated with pectate lyase, beta-elimination occurred, resulting in a double bond between C-4 and C-5 in the galacturonic acid residue of the released nonreducing end. The formation of double bonds produced an increase in light absorption, which was detected at 235 nm. The assay was tested on pectin of different origins (apple, orange, sugar beet, sunflower, celery, lemon), pectin derivatives (amidated pectin), and speciality types such as low molecular weight and low %DE (degree of esterification, percentage of galacturonic acid groups esterified with methanol) pectin. The highest response was given by pectate (pectin with %DE< 5) and the lowest by pectin extracted from sugar beet. No other gums (carboxymethylcellulose, carrageenan, locust bean gum, tragacanth, gellan, tamarind, xanthan, amylogum, sodium alginate, or agar) gave any response. Members of IPPA (International Pectin Producers Association) have evaluated the validity of the assay in a ring test. All members of the Association were able to identify pectin from other gums in a blind test. The method can replace more laborious and ambiguous identification tests which exist today.
Authors:
K M Hansen; A B Truesen; J R Søderberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies    
Journal Detail:
Title:  Journal of AOAC International     Volume:  84     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2001 Nov-Dec
Date Detail:
Created Date:  2001-12-19     Completed Date:  2002-06-10     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1851-4     Citation Subset:  IM    
Affiliation:
CP Kelco ApS, Lille Skensved, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Aspergillus niger / enzymology
Food Additives / analysis*
Food Analysis / methods*,  statistics & numerical data
Pectins / analysis*
Polysaccharide-Lyases
Recombinant Proteins
Chemical
Reg. No./Substance:
0/Food Additives; 0/Pectins; 0/Recombinant Proteins; 9000-69-5/pectin; EC 4.2.2.-/Polysaccharide-Lyases; EC 4.2.2.2/pectate lyase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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