Document Detail

Enzyme analysis of amniotic fluid for prenatal diagnosis of cystic fibrosis in high-risk pregnancies.
MedLine Citation:
PMID:  2904307     Owner:  NLM     Status:  MEDLINE    
We determined the activity concentrations of alkaline phosphatase (ALP), ALP isoenzymes, gamma-glutamyltransferase (GGT), and alpha-glucosidase (AGL) in 1200 unselected amniotic fluids and in amniotic fluids from 40 pregnancies at high risk for cystic fibrosis (CF). From the results we established the normal range and CF-predictive cutoff values for these enzymes in the second trimester of pregnancy. In all predicted normal pregnancies that went to term, normal children were born. Among the predicted affected pregnancies, 14 were terminated and two went to term, one resulting in a CF-affected child and the other in a healthy child. Evidence for CF was found in all 13 aborted fetuses examined (the parents of one refused to allow autopsy). We noted no differences in the amniotic fluid enzyme activities for the Arab and various Jewish ethnic groups living in Israel. We conclude that prenatal diagnosis of CF among the Israeli population at risk for CF is feasible by means of a reliable, fast, and economic test in the second trimester of pregnancy.
H Peretz; J Chemke; S Usher; C Legum; E Graff
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  34     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1988 Dec 
Date Detail:
Created Date:  1989-01-19     Completed Date:  1989-01-19     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2401-5     Citation Subset:  IM    
Department of Chemical Pathology, Tel-Aviv University, Israel.
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MeSH Terms
Alkaline Phosphatase / analysis
Amniotic Fluid / enzymology*
Clinical Enzyme Tests
Cystic Fibrosis / diagnosis*
Isoenzymes / analysis
Prenatal Diagnosis*
alpha-Glucosidases / analysis
gamma-Glutamyltransferase / analysis
Reg. No./Substance:
0/Isoenzymes; EC; EC Phosphatase; EC

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