| Enzyme amplified immunoassays. | |
| | |
MedLine Citation:
|
PMID: 2558597 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Enzyme amplification has been applied to improve the sensitivity of many immunoassays which use alkaline phosphatase as the label. The activity of the enzyme is amplified by using NAD, derived by hydrolysis of NADP, to activate catalytically a substrate cycle that generates a coloured product. In routine use, an amplification factor of 100-fold is easily achieved and, with longer incubation times, this can be increased to allow the detection of as few as 3,000 enzyme molecules. In order to avoid the limitations of spectrophotometry, an electrochemical version of the amplifier has recently been developed which performs with comparable sensitivity in a prototype device. The many advantages of electrochemical detection may ultimately herald the arrival of a new generation of simple, sensitive and inexpensive diagnostic systems. |
| | |
Authors:
|
D L Bates |
Related Documents
:
|
17360857 - Galactoxylomannan does not exhibit cross-reactivity in the platelia aspergillus enzyme ... 6189707 - Specific enzyme immunoassay for human chorionic gonadotropin using antibody to the synt... 10585347 - Clinical significance of immunoassays for type-5 tartrate-resistant acid phosphatase. 19362207 - Investigation of voltammetric enzyme-linked immunoassay system based on n-heterocyclic ... 568487 - Sex-related differences in diurnal activities and development of hepatic microsomal 3-h... 7575097 - Purification and properties of benzyl alcohol dehydrogenase from a denitrifying thauera... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Annales de biologie clinique Volume: 47 ISSN: 0003-3898 ISO Abbreviation: Ann. Biol. Clin. (Paris) Publication Date: 1989 |
Date Detail:
|
Created Date: 1990-02-16 Completed Date: 1990-02-16 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2984690R Medline TA: Ann Biol Clin (Paris) Country: FRANCE |
Other Details:
|
Languages: eng Pagination: 527-32 Citation Subset: IM |
Affiliation:
|
Novo BioLabs Ltd., Cambridge, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alkaline Phosphatase
/
metabolism* Electrochemistry Enzyme Activation* Humans Immunoassay / methods* Immunoenzyme Techniques / standards* Phosphoric Monoester Hydrolases Thyrotropin |
| Chemical | |
Reg. No./Substance:
|
9002-71-5/Thyrotropin; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Spectrophotometric determination of penicilloates in penicillins.
Next Document: The development of a novel immunoassay amplification system and its use in viral detection.