Document Detail


Enzyme amplified immunoassays.
MedLine Citation:
PMID:  2558597     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Enzyme amplification has been applied to improve the sensitivity of many immunoassays which use alkaline phosphatase as the label. The activity of the enzyme is amplified by using NAD, derived by hydrolysis of NADP, to activate catalytically a substrate cycle that generates a coloured product. In routine use, an amplification factor of 100-fold is easily achieved and, with longer incubation times, this can be increased to allow the detection of as few as 3,000 enzyme molecules. In order to avoid the limitations of spectrophotometry, an electrochemical version of the amplifier has recently been developed which performs with comparable sensitivity in a prototype device. The many advantages of electrochemical detection may ultimately herald the arrival of a new generation of simple, sensitive and inexpensive diagnostic systems.
Authors:
D L Bates
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Annales de biologie clinique     Volume:  47     ISSN:  0003-3898     ISO Abbreviation:  Ann. Biol. Clin. (Paris)     Publication Date:  1989  
Date Detail:
Created Date:  1990-02-16     Completed Date:  1990-02-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984690R     Medline TA:  Ann Biol Clin (Paris)     Country:  FRANCE    
Other Details:
Languages:  eng     Pagination:  527-32     Citation Subset:  IM    
Affiliation:
Novo BioLabs Ltd., Cambridge, UK.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism*
Electrochemistry
Enzyme Activation*
Humans
Immunoassay / methods*
Immunoenzyme Techniques / standards*
Phosphoric Monoester Hydrolases
Thyrotropin
Chemical
Reg. No./Substance:
9002-71-5/Thyrotropin; EC 3.1.3.-/Phosphoric Monoester Hydrolases; EC 3.1.3.1/Alkaline Phosphatase

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