| Enzyme Immunoassay for Tenuazonic Acid in Apple and Tomato Products. | |
| | |
MedLine Citation:
|
PMID: 22054343 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
The Alternaria mycotoxin tenuazonic acid was derivatized with succinic anhydride and conjugated to keyhole limpet hemocyanin (KLH) and to horseradish peroxidase (HRP), respectively. The KLH conjugate was used to produce polyclonal antibodies in rabbits. A competitive direct enzyme immunoassay (EIA) for tenuazonic acid was established, which was moderately sensitive for tenuazonic acid (50% inhibition concentration (IC50): 320 ± 130 ng/mL), but strongly reacted with tenuazonic acid acetate (IC50: 23.3 ± 7.5 ng/mL). Therefore an optimized EIA protocol was established, which employed acetylation of standard and sample extract solutions. The mean standard curve detection limit (IC30) for tenuazonic acid acetate was 5.4 ± 2.0 ng/mL, enabling detection limits for tenuazonic acid in apple and tomato products of 25 50 ng/g (150 ng/g in tomato paste). Recoveries in a concentration range of 50-2000 ng/g were 60-130% in apple juice and tomato juice, and 40-150% in other tomato products. Tenuazonic acid was detected in apple juice and tomato products from German retail shops at levels of 50-200 ng/g. In conclusion, this novel EIA for tenuazonic acid could be useful within a screening programme for Alternaria mycotoxins in food. |
| | |
Authors:
|
Madeleine Gross; Valeriu Curtui; Yvonne Ackermann; Hadri Latif; Ewald Usleber |
Related Documents
:
|
2266203 - Glycoproteins in the heat-and acid-stable fraction of ovine milk. 11084043 - Construction of acetate auxotrophs of neisseria meningitidis to study host-meningococca... 11996603 - Generalized principle of designing neutral superstrong brønsted acids. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-11-4 |
Journal Detail:
|
Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: - Publication Date: 2011 Nov |
Date Detail:
|
Created Date: 2011-11-7 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Spirituality in general practice: a qualitative evidence synthesis.
Next Document: A pilot study to explore the effectiveness of "early" rehabilitation after a hospital admission for ...