Document Detail

Enzymatically active papain preferentially induces an allergic response in mice.
MedLine Citation:
PMID:  9918815     Owner:  NLM     Status:  MEDLINE    
Human exposure to papain, a cysteine proteinase, is associated with hypersensitivity reactions. We demonstrate in mice that enzymatically active papain preferentially induces an IgG1 response and results in mast cell degranulation, both features typical of an allergic reaction. Inactive papain, blocked with E-64, appears to desensitize mice to subsequent challenge by active enzyme. These results suggest that the enzymatic activity of papain is critical in inducing an allergic response and that the use of inactive allergens may be a possible strategy for desensitizing allergic individuals.
L Chambers; A Brown; D I Pritchard; S Sreedharan; K Brocklehurst; N A Kalsheker
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  253     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-02-09     Completed Date:  1999-02-09     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  837-40     Citation Subset:  IM    
Division of Clinical Chemistry, School of Clinical Laboratory Sciences, University Hospital, Nottingham, England.
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MeSH Terms
Cell Degranulation
Cysteine Proteinase Inhibitors / pharmacology
Immunoglobulin G / blood
Leucine / analogs & derivatives,  pharmacology
Mast Cells / immunology
Mice, Inbred BALB C
Papain / antagonists & inhibitors,  immunology*,  isolation & purification
Reg. No./Substance:
0/Cysteine Proteinase Inhibitors; 0/Immunoglobulin G; 61-90-5/Leucine; 66701-25-5/E 64; EC

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