Document Detail


Enzymatically active colloidal crystal arrays.
MedLine Citation:
PMID:  18995867     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the construction of three-dimensional (3D) colloidal crystal arrays (CCA) and hollow colloidal crystal arrays (HCCA) derived from the self-assembly of polyelectrolytes (PE)-coated polystyrene (PS) particles and their use as models of high surface area systems to immobilize peroxidase (POD). POD molecules could infiltrate into the deep layers of CCA and HCCA through their interconnected pores and strongly adsorbed at the PE shell of the colloidal particles. And the total enzyme loading amount and bioactivities increased linearly with the thickness of the CCA till ca. 10 mum. Compared with flat substrates with the same geometrical area, CCA and HCCA exhibit much higher enzyme loading abilities (approximately 43 and 53 times respectively) and the resulting bioactivities (approximately 35 and 41 times respectively) due to their inherently higher surface area and 3D interconnected porous structures. In addition, HCCA could load approximately 30% more POD than CCA because some POD molecules could infiltrate into the interior of the hollow capsule under salt condition.
Authors:
Aimin Yu; Zhijian Liang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-10-17
Journal Detail:
Title:  Journal of colloid and interface science     Volume:  330     ISSN:  1095-7103     ISO Abbreviation:  J Colloid Interface Sci     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2008-12-01     Completed Date:  2009-02-09     Revised Date:  2009-11-11    
Medline Journal Info:
Nlm Unique ID:  0043125     Medline TA:  J Colloid Interface Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  144-8     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Murdoch University, Murdoch, 6150, Australia. a.yu@murdoch.edu.au
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MeSH Terms
Descriptor/Qualifier:
Capsules / chemistry
Colloids / chemistry*
Enzymes, Immobilized*
Peroxidase*
Polystyrenes
Chemical
Reg. No./Substance:
0/Capsules; 0/Colloids; 0/Enzymes, Immobilized; 0/Polystyrenes; EC 1.11.1.7/Peroxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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