Document Detail

Enzymatically cross-linked alginic-hyaluronic acid composite hydrogels as cell delivery vehicles.
MedLine Citation:
PMID:  23357799     Owner:  NLM     Status:  MEDLINE    
An injectable composite gel was developed from alginic and hyaluronic acid. The enzymatically cross-linked injectable gels were prepared via the oxidative coupling of tyramine modified sodium algiante and sodium hyaluronate in the presence of horse radish peroxidase (HRP) and hydrogen peroxide (H2O2). The composite gels were prepared by mixing equal parts of the two tyraminated polymer solutions in 10U HRP and treating with 1.0% H2O2. The properties of the alginate gels were significantly affected by the addition of hyaluronic acid. The percentage water absorption and storage modulus of the composite gels were found to be lower than the alginate gels. The alginate and composite gels showed lower protein release compared to hyaluronate gels in the absence of hyaluronidase. Even hyaluronate gels showed only approximately 10% protein release after 14 days incubation in phosphate buffer solution. ATDC-5 cells encapsulated in the injectable gels showed high cell viability. The composite gels showed the presence of enlarged spherical cells with significantly higher metabolic activity compared to cells in hyaluronic and alginic acid gels. The results suggest the potential of the composite approach to develop covalently cross-linked hydrogels with tuneable physical, mechanical, and biological properties.
Nitya Ganesh; Craig Hanna; Shantikumar V Nair; Lakshmi S Nair
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-26
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  55     ISSN:  1879-0003     ISO Abbreviation:  Int. J. Biol. Macromol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-11     Completed Date:  2013-08-23     Revised Date:  2014-04-02    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  289-94     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier B.V. All rights reserved.
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MeSH Terms
Alginates / chemistry*
Biocompatible Materials / chemistry
Cell Line
Cell Proliferation
Cell Survival
Glucuronic Acid / chemistry
Hexuronic Acids / chemistry
Hyaluronic Acid / chemistry*
Hydrogels / chemistry*
Tyramine / chemistry
Grant Support
Reg. No./Substance:
0/Alginates; 0/Biocompatible Materials; 0/Hexuronic Acids; 0/Hydrogels; 059QF0KO0R/Water; 8A5D83Q4RW/Glucuronic Acid; 9004-61-9/Hyaluronic Acid; 9005-32-7/alginic acid; X8ZC7V0OX3/Tyramine

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