Document Detail

Enzymatically Catalyzed Synthesis of Anti-blooming Agent 1,3-dibehenoyl-2-oleoyl glycerol in a Solvent-free System: Optimization by Response Surface Methodology.
MedLine Citation:
PMID:  24147905     Owner:  NLM     Status:  Publisher    
Products rich in 1,3-dibehenoyl-2-oleoyl glycerol (BOB) triglyceride (TAG) were produced by enzymatic interesterification of high oleic acid sunflower oil (HOSO) and behenic acid methyl ester (BME) by 1,3-regiospecific lipase Lipozyme RM IM in a solvent-free system. The impact factors of enzyme load, the substrate molar ratio of BME to HOSO (BME:HOSO), reaction time, reaction temperature, and pre-equilibration water activity of the enzyme on BOB content and BME conversions were investigated by single-factor experiments and then optimized using the response surface methodology (RSM). The optimum conditions were as follows: reaction temperature 72 ºC, reaction time 7.99 h, substrate molar ratio 2.5:1, enzyme load 10% and pre-equilibration water activities of the enzyme 0.28. The results from the experiments which conducted according to the predicted optimal conditions were as follows: the content of BOB was 32.76%; the conversion of BME was 65.16%. The experimental values were agreeing with the predicted values, which verified the sufficiency of the quadratic regression models. After purification under the optimal short-range molecular distillation and two-step solvent fractionation, the content of BOB in the target product can reach 77.14%, indicating the great potential for industrial production of the anti-blooming agent.
Zong Meng; Wenxin Geng; Jinwei Li; Zhaoqi Yang; Jiang Jiang; Xingguo Wang; Yuanfa Liu
Related Documents :
24136565 - Screening and characterization of protease producing actinomycetes from marine saltern.
3478705 - Reversal of age-related effects in rat muscle phosphoglycerate kinase.
22994585 - Inhibitory role of monovalent ions on rat brain cortex adenylyl cyclase activity.
23594205 - Complex formation and regulation of escherichia coli acetyl-coa carboxylase.
5105 - The effects of grass and concentrate diets on the specific activities of some enzymes o...
6894155 - Dissociation and unfolding of sulfhydryl oxidase in solutions of guanidinium chloride.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-10-22
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-10-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  In Vitro Evaluation of an External Compression Device for Fontan Mechanical Assistance.
Next Document:  Short Antibacterial Peptides with Significantly Reduced Hemolytic Activity can be Identified by a Sy...