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Enzymatically Catalyzed Synthesis of Anti-blooming Agent 1,3-dibehenoyl-2-oleoyl glycerol in a Solvent-free System: Optimization by Response Surface Methodology.
MedLine Citation:
PMID:  24147905     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Products rich in 1,3-dibehenoyl-2-oleoyl glycerol (BOB) triglyceride (TAG) were produced by enzymatic interesterification of high oleic acid sunflower oil (HOSO) and behenic acid methyl ester (BME) by 1,3-regiospecific lipase Lipozyme RM IM in a solvent-free system. The impact factors of enzyme load, the substrate molar ratio of BME to HOSO (BME:HOSO), reaction time, reaction temperature, and pre-equilibration water activity of the enzyme on BOB content and BME conversions were investigated by single-factor experiments and then optimized using the response surface methodology (RSM). The optimum conditions were as follows: reaction temperature 72 ºC, reaction time 7.99 h, substrate molar ratio 2.5:1, enzyme load 10% and pre-equilibration water activities of the enzyme 0.28. The results from the experiments which conducted according to the predicted optimal conditions were as follows: the content of BOB was 32.76%; the conversion of BME was 65.16%. The experimental values were agreeing with the predicted values, which verified the sufficiency of the quadratic regression models. After purification under the optimal short-range molecular distillation and two-step solvent fractionation, the content of BOB in the target product can reach 77.14%, indicating the great potential for industrial production of the anti-blooming agent.
Authors:
Zong Meng; Wenxin Geng; Jinwei Li; Zhaoqi Yang; Jiang Jiang; Xingguo Wang; Yuanfa Liu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-10-22
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2013 Oct 
Date Detail:
Created Date:  2013-10-23     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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