Document Detail


Enzymatic synthesis of L-tryptophan from hair acid hydrolysis industries wastewater with tryptophan synthase.
MedLine Citation:
PMID:  20884203     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
An effective method for production of L-tryptophan from hair acid hydrolysis wastewater (HHW) containing L-serine was developed by recombinant tryptophan synthase. This study provides us with an alternative HHW utilization strategy. Tryptophan synthase could efficiently convert L-serine contained in HHW to L-tryptophan at pH 8.0, 40°C and Tween-80 of 0.04%. The enzyme also showed high tolerance to ammonium chloride, a component in HHW. In a scale up study, L-serine conversion rate reach 95.1% with a final L-tryptophan concentration of 33.2 g l(-1).
Authors:
Genhai Zhao; Junzhong Liu; Kai Dong; Fei Zhang; Hongjuan Zhang; Qian Liu; Qingcai Jiao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-21
Journal Detail:
Title:  Bioresource technology     Volume:  102     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3554-7     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, People's Republic of China.
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