Document Detail

Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372.
MedLine Citation:
PMID:  18800597     Owner:  NLM     Status:  MEDLINE    
A purified alkaline thermo-tolerant bacterial lipase from Bacillus cereus MTCC 8372 was immobilized on a Poly (MAc-co-DMA-cl-MBAm) hydrogel. The hydrogel showed approximately 94% binding capacity for lipase. The immobilized lipase (2.36 IU) was used to achieve esterification ofmyristic acid and isopropanol in n-heptane at 65 degrees C under continuous shaking. The myristic acid and isopropanol when used at a concentration of 100 mM each in n-heptane resulted in formation of isopropyl myristate (66.0 +/- 0.3 mM) in 15 h. The reaction temperature below or higher than 65 degrees C markedly reduced the formation of isopropyl myristate. Addition of a molecular sieve (3 A x 1.5 mm) to the reaction mixture drastically reduced the ester formation. The hydrogel bound lipase when repetitively used to perform esterification under optimized conditions resulted in 38.0 +/- 0.2 mM isopropyl myristate after the 3rd cycle of esterification.
M L Verma; G S Chauhan; S S Kanwar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta microbiologica et immunologica Hungarica     Volume:  55     ISSN:  1217-8950     ISO Abbreviation:  Acta Microbiol Immunol Hung     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-19     Completed Date:  2008-10-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9434021     Medline TA:  Acta Microbiol Immunol Hung     Country:  Hungary    
Other Details:
Languages:  eng     Pagination:  327-42     Citation Subset:  IM    
Department of Biotechnology, Himachal Pradesh University, Summer-Hill, Shimla 171 005, India.
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MeSH Terms
2-Propanol / metabolism
Bacillus cereus / enzymology*
Enzymes, Immobilized / metabolism*
Heptanes / metabolism
Lipase / metabolism*
Molecular Structure
Myristates / metabolism*
Myristic Acid / metabolism
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Heptanes; 0/Myristates; 110-27-0/isopropyl myristate; 142-82-5/n-heptane; 25852-47-5/Hydrogel; 544-63-8/Myristic Acid; 67-63-0/2-Propanol; EC

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