| Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372. | |
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MedLine Citation:
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PMID: 18800597 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A purified alkaline thermo-tolerant bacterial lipase from Bacillus cereus MTCC 8372 was immobilized on a Poly (MAc-co-DMA-cl-MBAm) hydrogel. The hydrogel showed approximately 94% binding capacity for lipase. The immobilized lipase (2.36 IU) was used to achieve esterification ofmyristic acid and isopropanol in n-heptane at 65 degrees C under continuous shaking. The myristic acid and isopropanol when used at a concentration of 100 mM each in n-heptane resulted in formation of isopropyl myristate (66.0 +/- 0.3 mM) in 15 h. The reaction temperature below or higher than 65 degrees C markedly reduced the formation of isopropyl myristate. Addition of a molecular sieve (3 A x 1.5 mm) to the reaction mixture drastically reduced the ester formation. The hydrogel bound lipase when repetitively used to perform esterification under optimized conditions resulted in 38.0 +/- 0.2 mM isopropyl myristate after the 3rd cycle of esterification. |
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Authors:
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M L Verma; G S Chauhan; S S Kanwar |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta microbiologica et immunologica Hungarica Volume: 55 ISSN: 1217-8950 ISO Abbreviation: Acta Microbiol Immunol Hung Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-19 Completed Date: 2008-10-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9434021 Medline TA: Acta Microbiol Immunol Hung Country: Hungary |
Other Details:
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Languages: eng Pagination: 327-42 Citation Subset: IM |
Affiliation:
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Department of Biotechnology, Himachal Pradesh University, Summer-Hill, Shimla 171 005, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2-Propanol
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metabolism Bacillus cereus / enzymology* Enzymes, Immobilized / metabolism* Heptanes / metabolism Hydrogel Kinetics Lipase / metabolism* Molecular Structure Myristates / metabolism* Myristic Acid / metabolism Temperature |
| Chemical | |
Reg. No./Substance:
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0/Enzymes, Immobilized; 0/Heptanes; 0/Myristates; 110-27-0/isopropyl myristate; 142-82-5/n-heptane; 25852-47-5/Hydrogel; 544-63-8/Myristic Acid; 67-63-0/2-Propanol; EC 3.1.1.3/Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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