| Enzymatic synthesis of Q nucleoside containing mannose in the anticodon of tRNA: isolation of a novel mannosyltransferase from a cell-free extract of rat liver. | |
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MedLine Citation:
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PMID: 20603 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Q nucleosides isolated from rabbit liver tRNA are known to have sugars (mannose or galactose) linked to their cyclopentene diol moiety. A Q nucleoside containing mannose (manQ) was synthesized by a cell-free system from rat liver, using purified E. coli tRNAAsp as an acceptor and GDP-mannose as a donor molecule. The novel mannosyltransferase catalyzing this reaction was purified from a particulate-free soluble enzyme fraction and found to be strictly specific for tRNAAsp. These results, together with the anomeric configuration of mannose in Q nucleoside, indicate that no lipid intermediate is involved in the biosynthesis of Q nucleoside. |
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Authors:
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N Okada; S Nishimura |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nucleic acids research Volume: 4 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1977 Aug |
Date Detail:
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Created Date: 1977-11-30 Completed Date: 1977-11-30 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 2931-8 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticodon Edetic Acid / pharmacology Hexosyltransferases / metabolism* Hydrogen-Ion Concentration Kinetics Liver / enzymology* Magnesium / pharmacology Male Mannosyltransferases / isolation & purification, metabolism* RNA, Transfer* / biosynthesis Rats Ribonucleosides / biosynthesis* Sodium Chloride / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Anticodon; 0/Ribonucleosides; 60-00-4/Edetic Acid; 7439-95-4/Magnesium; 7647-14-5/Sodium Chloride; 9014-25-9/RNA, Transfer; EC 2.4.1.-/Hexosyltransferases; EC 2.4.1.-/Mannosyltransferases |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  ? Information Retrieval Limited 1 Falconberg Court London W1V 5FG England Print publication date: Month: 08 Year: 1977 Volume: 4 Issue: 8 First Page: 2931 Last Page: 2937 ID: 342620 PubMed Id: 20603 |
| Enzymatic synthesis of Q* nucleoside containing mannose in the anticodon of tRNA: isolation of a novel mannosyltransferase from a cell-free extract of rat liver | |
| Norihiro Okada | |
| Susumu Nishimura | |
| Biology Division, National Cancer Center Research Institute, Tsukiji 5-1, Chuo-ku, Tokyo, Japan |
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