Document Detail

Enzymatic synthesis of β-N-(γ-L: (+)-glutamyl)phenylhydrazine with Escherichia coli γ-glutamyltranspeptidase.
MedLine Citation:
PMID:  22791224     Owner:  NLM     Status:  Publisher    
A new method for the synthesis of β-N-(γ-L: (+)-glutamyl)phenylhydrazine is presented. This compound was prepared from L: -glutamine and phenylhydrazine through a transpeptidation reaction of Escherichia coli γ-glutamyltranspeptidase although phenylhydrazine has been reported to be an inhibitor of the enzyme. The optimum reaction conditions were 60 mM L: -glutamine, 300 mM phenylhydrazine, 40 U γ-glutamyltranspeptidase/ml, and pH 9 in approx. 800 ml. After 6 h at 37 °C, the product was obtained with a conversion rate of 93 % (mol/mol). γ-Glutamyltranspeptidase was reversibly inhibited only when phenylhydrazine was above 300 mM.
Hongjuan Zhang; Yueping Zhan; Junjun Chang; Junzhong Liu; Lisheng Xu; Zhiyuan Wang; Qian Liu; Qingcai Jiao
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-13
Journal Detail:
Title:  Biotechnology letters     Volume:  -     ISSN:  1573-6776     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, 210093, Jiangsu, China.
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