| Enzymatic synthesis of β-N-(γ-L: (+)-glutamyl)phenylhydrazine with Escherichia coli γ-glutamyltranspeptidase. | |
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MedLine Citation:
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PMID: 22791224 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A new method for the synthesis of β-N-(γ-L: (+)-glutamyl)phenylhydrazine is presented. This compound was prepared from L: -glutamine and phenylhydrazine through a transpeptidation reaction of Escherichia coli γ-glutamyltranspeptidase although phenylhydrazine has been reported to be an inhibitor of the enzyme. The optimum reaction conditions were 60 mM L: -glutamine, 300 mM phenylhydrazine, 40 U γ-glutamyltranspeptidase/ml, and pH 9 in approx. 800 ml. After 6 h at 37 °C, the product was obtained with a conversion rate of 93 % (mol/mol). γ-Glutamyltranspeptidase was reversibly inhibited only when phenylhydrazine was above 300 mM. |
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Authors:
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Hongjuan Zhang; Yueping Zhan; Junjun Chang; Junzhong Liu; Lisheng Xu; Zhiyuan Wang; Qian Liu; Qingcai Jiao |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-13 |
Journal Detail:
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Title: Biotechnology letters Volume: - ISSN: 1573-6776 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, 210093, Jiangsu, China. |
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