Document Detail

Enzymatic, spectrophotometric determination of glucose, fructose, sucrose, and inulin/oligofructose in foods.
MedLine Citation:
PMID:  15493679     Owner:  NLM     Status:  MEDLINE    
A fast, simple, and accurate method, using only standard laboratory equipment, was developed for the quantification of glucose, fructose, sucrose, and inulin/oligofructose in different food matrixes. Samples were extracted using boiling water and hydrolyzed with sucrase and fructanase. Sugars were determined in the initial extract and in both hydrolysates using an enzymatic, spectrophotometric kit for glucose and fructose determination with hexokinase, glucose-6-phosphate dehydrogenase, and phosphoglucose isomerase. Calculations of sucrose and inulin/oligofructose were based only on fructose measurement. Glucose results of the hydrolysates were not used for inulin/oligofructose calculations because of possible interference. Released glucose by the hydrolysis of maltose or by possible partial hydrolysis of other compounds like maltodextrines, starch, lactose, or maltitol could interfere in the measurement of the sucrase and the fructanase hydrolysates. To validate the method, a wide range of different food matrixes and different amounts of inulin/oligofructose (1-54%) were analyzed. Mean recovery +/- relative standard deviation (RSD) for inulin or oligofructose was 96.0 +/- 5.3%. The RSDr for inulin/oligofructose measured on 35 food samples, analyzed in duplicate, was 5.9%. Accuracy and precision of the method were less for samples with large concentrations of sucrose, maltose, maltodextrines, or starch (ratio to inulin/oligofructose >4 to 1). Precision and accuracy were comparable with those of the ion exchange chromatographic method AOAC 997.08 and the enzymatic, spectrophotometric method AOAC 999.03. In contrast to 999.03, this method allows the accurate quantification of both GFn and Fn forms.
Monique Steegmans; Saskia Iliaens; Hubert Hoebregs
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  87     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2004 Sep-Oct
Date Detail:
Created Date:  2004-10-20     Completed Date:  2005-01-27     Revised Date:  2008-03-17    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1200-7     Citation Subset:  IM    
Orafti, Analytical Services, Aandorenstraat 1, B-3300 Tienen, Belgium.
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MeSH Terms
Food Analysis*
Fructose / analysis*
Glucose / analysis*
Inulin / analysis*
Oligosaccharides / analysis*
Reagent Kits, Diagnostic
Sucrose / analysis*
Reg. No./Substance:
0/Oligosaccharides; 0/Reagent Kits, Diagnostic; 0/oligofructose; 30237-26-4/Fructose; 50-99-7/Glucose; 57-50-1/Sucrose; 9005-80-5/Inulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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