| Enzymatic reaction of beta-N-methylaminoalanine with L-amino acid oxidase. | |
| | |
MedLine Citation:
|
PMID: 2043677 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The reaction of beta-N-methylaminoalanine (BMAA) with L-amino acid oxidase (L-AAO) in the presence of catalase yields ammonia and beta-N-methylaminopyruvate, which was trapped as its 2,4-dinitrophenylhydrazone, as products. Incubation of BMAA with L-AAO in the presence of semicarbazide led to the formation of a semicarbazone, indicating intermediate iminium ion formation; when potassium cyanide (5 mM) was added, semicarbazone formation was blocked. The formation of beta-N-methylaminopyruvate was decreased by omission of catalase and was reduced in the presence of hydrogen peroxide (100 mM). These results indicate that BMAA is converted by L-AAO to the corresponding alpha-imino acid, which undergoes hydrolysis to beta-N-methylaminopyruvate. The alpha-keto acid is readily oxidized to N-methylglycine by hydrogen peroxide. |
| | |
Authors:
|
M Hashmi; M W Anders |
Related Documents
:
|
19256537 - New triterpenic saponins from the aerial parts of medicago arabica (l.) huds. 11197157 - Evaluation of norbornene- beta-cyclodextrin-based monomers and oligomers as chiral sele... 9124397 - Taurine reabsorption by a carrier interacting with furosemide in short and long henle's... 19128027 - A new strategy for the synthesis of optically pure beta-fluoroalkyl beta-amino acid der... 21148477 - Dietary fat sensing via fatty acid oxidation in enterocytes - possible role in the cont... 8323967 - Specific labeling of candida tropicalis peroxisomal proteins with photoreactive fatty-a... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 1074 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1991 May |
Date Detail:
|
Created Date: 1991-07-17 Completed Date: 1991-07-17 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 36-9 Citation Subset: IM |
Affiliation:
|
Department of Pharmacology, University of Rochester, School of Medicine and Dentistry, NY 14642. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Oxidoreductases
/
chemistry* Amino Acids, Diamino / chemistry* Carcinogens / chemistry Catalase / chemistry Kinetics L-Amino Acid Oxidase Molecular Structure Potassium Cyanide / chemistry Semicarbazides / chemistry |
| Grant Support | |
ID/Acronym/Agency:
|
ES01247/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids, Diamino; 0/Carcinogens; 0/Semicarbazides; 151-50-8/Potassium Cyanide; 16676-91-8/alpha-amino-beta-methylaminopropionate; 57-56-7/carbamylhydrazine; EC 1.11.1.6/Catalase; EC 1.4.-/Amino Acid Oxidoreductases; EC 1.4.3.2/L-Amino Acid Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Spermidine N1-acetyltransferase activity in rat uterus after 17 beta-estradiol and during the estrou...
Next Document: Cholinesterase solubilizing factor from Cytophaga sp. is a phosphatidylinositol-specific phospholipa...