| Enzymatic properties of polyethylene glycol-modified cholesterol esterase in organic solvents. | |
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MedLine Citation:
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PMID: 1418688 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The solvent dependency and substrate specificity of polyethylene glycol (PEG)-modified cholesterol esterase (CEH) catalyzing cholesterol ester synthesis in organic solvents were studied. When cholesterol and linoleic acid were used as the substrates, PEG-modified CEH synthesized cholesterol linoleate only in water-immiscible organic solvents. Among some solvents capable of solubilizing all of the reaction components (PEG-modified CEH, cholesterol, and linoleic acid), chloroform was most suitable for enzymatic cholesterol linoleate synthesis, and the synthetic activity for cholesterol linoleate decreased in the order chloroform, benzene, toluene, and cyclohexane. PEG-modified CEH synthesized various cholesterol esters with significant substrate specificity. The substrate specificity for cholesterol ester synthesis in benzene was analogous to that for cholesterol ester hydrolysis in aqueous solution. |
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Authors:
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S Mori; Y Nakata; H Endo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biotechnology and applied biochemistry Volume: 16 ISSN: 0885-4513 ISO Abbreviation: Biotechnol. Appl. Biochem. Publication Date: 1992 Aug |
Date Detail:
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Created Date: 1992-12-18 Completed Date: 1992-12-18 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8609465 Medline TA: Biotechnol Appl Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 101-5 Citation Subset: IM |
Affiliation:
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School of Health Sciences, Okayama University, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cholesterol Linoleic Acid Linoleic Acids Polyethylene Glycols Pseudomonas / enzymology Solvents Sterol Esterase / chemistry, metabolism* Streptomyces / enzymology Substrate Specificity |
| Chemical | |
Reg. No./Substance:
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0/Linoleic Acids; 0/Polyethylene Glycols; 0/Solvents; 2197-37-7/Linoleic Acid; 57-88-5/Cholesterol; EC 3.1.1.13/Sterol Esterase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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