| Enzymatic properties of human cytosolic phospholipase A(2)gamma. | |
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MedLine Citation:
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PMID: 12039969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The enzymatic properties of cytosolic phospholipase A(2)gamma (cPLA(2)gamma), an isoform of 85-kDa group IV cPLA(2)alpha (cPLA(2)alpha) were studied in vitro and when the enzyme was expressed in cells. cPLA(2)gamma expressed in Sf9 cells is associated with membrane. Membranes isolated from [(3)H]arachidonic acid-labeled Sf9 cells expressing cPLA(2)gamma, constitutively release [(3)H]arachidonic acid. The membrane-associated activity is inhibited by the group IV PLA(2) inhibitor methylarachidonyl fluorophosphonate, but not effectively by the group VI PLA(2) inhibitor (E)-6-(bromomethylene)-3-(1-naphthalenyl)-2H-tetrahydropyran-2-one. cPLA(2)gamma has higher lysophospholipase activity than PLA(2) activity. Purified His-cPLA(2)gamma does not exhibit phospholipase A(1) activity, but sequentially hydrolyzes fatty acid from the sn-2 and sn-1 positions of phosphatidylcholine. cPLA(2)gamma overexpressed in HEK293 cells is constitutively active in isolated membranes, releasing large amounts of oleic, arachidonic, palmitic, and stearic acids; however, basal fatty acid release from intact cells is not increased. cPLA(2)gamma overexpressed in lung fibroblasts from cPLA(2)alpha-deficient mice is activated by mouse serum resulting in release of arachidonic, oleic, and palmitic acids, whereas overexpression of cPLA(2)alpha results primarily in arachidonic acid release. |
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Authors:
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Allison Stewart; Moumita Ghosh; Diane M Spencer; Christina C Leslie |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. Date: 2002-05-30 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 277 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-08-12 Completed Date: 2002-09-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 29526-36 Citation Subset: IM |
Affiliation:
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Program in Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, Colorado 80206, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Cytosol / enzymology* DNA Primers Enzyme Inhibitors / pharmacology Fatty Acids / metabolism Humans Phospholipases A / antagonists & inhibitors, genetics, metabolism* Recombinant Proteins / genetics, metabolism Spodoptera |
| Grant Support | |
ID/Acronym/Agency:
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HL34303/HL/NHLBI NIH HHS; HL61378/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Enzyme Inhibitors; 0/Fatty Acids; 0/Recombinant Proteins; EC 3.1.1.-/Phospholipases A |
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