Document Detail


Enzymatic production and characterization of manno-oligosaccharides from Gleditsia sinensis galactomannan gum.
MedLine Citation:
PMID:  23357797     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Enzymatic hydrolysis of Gleditsia sinensis gum was performed to produce manno-oligosaccharides having functional applications as dietary fiber and prebiotics. The optimum hydrolysis conditions, including enzyme loading, temperature and time, from response surface methodology were 8.1 U/g, 57.4°C and 34.1h, respectively. The yield of DP 1-5 oligosaccharides was 75.9% (29.1g/L). The Michaelis-Menten kinetics and molecular weight distribution were determined. The obtained oligosaccharides were further separated by HPLC and SEC, and the galactose distribution of G. sinensis gum was elucidated. Results indicated that G. sinensis gum has potential to produce value-added oligosaccharides in food industries.
Authors:
Hong-Lei Jian; Li-Wei Zhu; Wei-Ming Zhang; Da-Feng Sun; Jian-Xin Jiang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-25
Journal Detail:
Title:  International journal of biological macromolecules     Volume:  -     ISSN:  1879-0003     ISO Abbreviation:  Int. J. Biol. Macromol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909578     Medline TA:  Int J Biol Macromol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013. Published by Elsevier B.V.
Affiliation:
Department of Chemistry and Chemical Engineering, Beijing Forestry University, Beijing, 100083, China.
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