Document Detail

Enzymatic preparation and purification of prostaglandin E2.
MedLine Citation:
PMID:  5689556     Owner:  NLM     Status:  MEDLINE    
An enzymatic system has been developed for the production of prostaglandin E(2) (PGE(2)) from arachidonic acid by extracts of sheep seminal vesicular glands. The presence of glutathione insures high yields. A new procedure for the purification of PGE(2) was also developed, based on the dialysis of the biosynthesized product at pH 8 and extraction of the dialysate at pH 3 with chloroform. This procedure routinely gives yields of PGE(2) of 25-37% (from arachidonic acid) with a purity of 90-100%. Additional analytical proof of the identity of PGE(2) was provided by physicochemical characteristics of the crystalline thiosemicarbazide derivative, which can be readily prepared under mild conditions.
M Lapidus; N H Grant; H E Alburn
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  9     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1968 May 
Date Detail:
Created Date:  1968-06-05     Completed Date:  1968-06-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  371-3     Citation Subset:  IM    
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MeSH Terms
Arachidonic Acids / metabolism*
Chromatography, Thin Layer
Glutathione / pharmacology
Infrared Rays
Prostaglandins / biosynthesis*
Seminal Vesicles / enzymology*,  metabolism
Spectrum Analysis
Tissue Extracts
Reg. No./Substance:
0/Arachidonic Acids; 0/Prostaglandins; 0/Thiosemicarbazones; 0/Tissue Extracts; 70-18-8/Glutathione

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