Document Detail


Enzymatic preparation and purification of prostaglandin E2.
MedLine Citation:
PMID:  5689556     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An enzymatic system has been developed for the production of prostaglandin E(2) (PGE(2)) from arachidonic acid by extracts of sheep seminal vesicular glands. The presence of glutathione insures high yields. A new procedure for the purification of PGE(2) was also developed, based on the dialysis of the biosynthesized product at pH 8 and extraction of the dialysate at pH 3 with chloroform. This procedure routinely gives yields of PGE(2) of 25-37% (from arachidonic acid) with a purity of 90-100%. Additional analytical proof of the identity of PGE(2) was provided by physicochemical characteristics of the crystalline thiosemicarbazide derivative, which can be readily prepared under mild conditions.
Authors:
M Lapidus; N H Grant; H E Alburn
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of lipid research     Volume:  9     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1968 May 
Date Detail:
Created Date:  1968-06-05     Completed Date:  1968-06-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  371-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acids / metabolism*
Chromatography, Thin Layer
Crystallization
Dialysis
Glutathione / pharmacology
Infrared Rays
Male
Prostaglandins / biosynthesis*
Seminal Vesicles / enzymology*,  metabolism
Sheep
Spectrum Analysis
Thiosemicarbazones
Tissue Extracts
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Prostaglandins; 0/Thiosemicarbazones; 0/Tissue Extracts; 70-18-8/Glutathione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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