Document Detail


Enzymatic pathway for biodegrading microcystin LR in Sphingopyxis sp. C-1.
MedLine Citation:
PMID:  22883536     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The mlr gene cluster consisting of mlrA, mlrB, mlrC, and mlrD is involved in the degradation of the cyanobacterial toxin microcystin. However, it is unclear which degradation intermediates are metabolized by MlrB and MlrC. To address these questions, we constructed recombinant Escherichia coli to overproduce MlrB and MlrC from Sphingopyxis sp. C-1, and determined which intermediates were degraded in cell-free extracts. The cell-free extract containing MlrB degraded linearized microcystin-LR, giving rise to a tetrapeptide. The cell-free extract of MlrC degraded linearized microcystin-LR and also degraded the tetrapeptide to the amino acid Adda. These results indicate that linearized microcystin-LR is degraded by both MlrB and MlrC, and tetrapeptide is degraded by specifically by MlrC in Sphingopyxis sp. C-1.
Authors:
Kazuya Shimizu; Hideaki Maseda; Kunihiro Okano; Takumi Kurashima; Yukio Kawauchi; Qiang Xue; Motoo Utsumi; Zhenya Zhang; Norio Sugiura
Related Documents :
215716 - Infection of human amnion cells with cytomegalovirus.
8794336 - Human cell receptor cd46 is down regulated through recognition of a membrane-proximal r...
2527966 - Role of adenovirus type 2 early region 1b 19k protein stability in expression of the cy...
6243206 - Replication of murine cytomegalovirus in reproductive tissues.
10757336 - Cytologic and histologic diagnosis and significance of controversial squamous lesions o...
7569316 - Electrophoretic mobility of external invertase from free and gel-immobilized yeast cells.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-8
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  -     ISSN:  1347-4421     ISO Abbreviation:  J. Biosci. Bioeng.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
Affiliation:
Faculty of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura, Ora-gun, Gunma 374-0193, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effects of dissolved oxygen on the biooxidation process of refractory gold ores.
Next Document:  Emerging perspectives in the restoration of biodiversity-based ecosystem services.